热休克蛋白47在瘢痕疙瘩组织中表达及与胶原蛋白的相关性研究

目的探讨热休克蛋白47（HSP47）在瘢痕疙瘩组织中的表达及与I型、Ⅲ型胶原的相关性。方法采用免疫组织化学染色方法检测2l例（瘢痕组）病理性瘢痕疙瘩组织中HSP47、I型、Ⅲ型胶原等水平，并与正常包皮组织（对照组）相比较，观察两组HSP47表达情况及I型和Ⅲ型胶原水平。结果瘢痕组HSP47吸光度值（A值）（133．0-~-58．7），明显低于对照组的（169．6±53．2）（t=2．697，P=0．024）；两组I型胶原、Ⅲ型胶原水平差异均有统计学意义（t=14．898、12．610，均P=0．000）；HSP47水平与I型胶原含量呈正相关（r=3．058，P=0．034），与Ⅲ型胶原含量也呈正相关（r=4．610，P=0．029）。结论HSP47在瘢痕疙瘩组织中沉积可引起真皮层中胶原蛋白的合成显著增多，是瘢痕疙瘩形成的一个重要机制。

Heat shock protein 47 with pathological correlation of collagen in keloid

Objective To study the heat shock protein 47 ( HSP 47 ) andtype Ⅰ ,Ⅲ collagen relevance expression in keloid tissue. Methods USE immunohistochemistry to detect 21 patients( HS group) pathological keloid tissue HSP47, Ⅰ-type, Ⅲ collagen levels,and normal foreskin(control group) compared to the expression of HSP 47 and Ⅰ and Ⅲ collagen. Results HS group HSP 47 scar absorbance was( A value) ( 133.0 ± 58.7) ,it was significantly lower than the control group( 169.6 ± 53.2) ( t =2. 697 ,P =0.024) ;two groups of collagen type Ⅰ, Ⅲ collagen levels had statistically differentce(t =14.898,12.610,all P =0.000) ; HSP 47 level and type Ⅰ collagen were positively correlated(r =3.058,P =0. 034),and type Ⅲ collagen content was also positively correlated(r =4. 610, P =0. 029). Conclusion HSP 47 in keloid tissue could cause dermal layer assembly of collagen synthesis and significantly increased,and it was an important mechanism for the formation of keloids.