微量全血真空采血管培养法应用于染色体畸变分析的研究

目的建立一种简便有效的外周血淋巴细胞染色体培养及畸变分析方法。方法以市售国产的真空采血管为培养载体,微量全血培养54 h,收获、制片、镜检并将染色体畸变分析结果与常规培养方法进行比较(χ2检验)。结果该体系对60名放射工作人员和30名正常对照人员的外周血淋巴细胞染色体畸变进行检测分析,发现本法与常规法差异无统计学意义(P>0.05)。结论微量全血真空采血管培养法可用于染色体的培养及畸变的检测,简便、有效,值得推广。

Study on Chromosome Aberrations Test determinated by Micro-whole Blood Culture in Vacuum Blood Collection Tube

Objective To develop an easier and efficient method of culturing the chromosome and analyzing the aberrations in peripheral lymphocytes.Methods Micro whole was cultured for 54 hours in home-made vacuum blood collection tube,and then collection,slice-making,microscopy detection for the chromosome aberrations was done.The difference of the results was analysed by comparing with the common method.Results For 60 radiologists and 30 contrasts,the chromosome aberrations in peripheral lymphocytes were examed by this system,the lymphocytes and chromosome were clear and alive and easier to analyse.Compared with the common method,there was no significantly difference between the two analyzing results.Conclusion The chromosome aberrations test by micro whole blood culture in vacuum blood collection tube is easier and efficient,and is worthy of being widely popularized.