基于HPLC指纹图谱技术对小包装熟地黄饮片的稳定性考察

目的:探讨不同包装材料、不同存贮状态对熟地黄饮片质量稳定性的影响,建立熟地黄最佳贮存养护方法.方法:分别采用3种不同包装材料将熟地黄贮存于3种不同温度及2种密闭环境中,采用HPLC指纹图谱技术,以熟地黄指标性成分毛蕊花糖苷为评价指标,评价不同包装材料、不同存贮状态对熟地黄饮片质量稳定性的影响.结果:PTP铝箔、真空、冷藏条件下毛蕊花糖苷含量最高分别为(0.028±0.011)％,(0.026±0.016)％,(0.025±0.014)％.毛蕊花糖苷含量高低顺序为PTP铝箔＞聚乙烯塑料袋＞牛皮凝膜纸;真空状态＞非真空状态;冷藏条件＞阴凉条件＞常温条件.熟地黄最佳贮存养护条件为PTP铝箔包装,真空冷藏.结论:该优选条件下,熟地黄饮片质量稳定,可为实际生产中贮存熟地黄提供试验依据.

Stability Investigation of Small Package Rehmannia glutinosa Pieces based on HPLC Fingerprint Technology

Objective:To investigate influence of different packaging materials and different storage state on quality stability of Rehmannia glutinosa pieces, and establish the best storage conservation method of R. glutinosa. Method:Three different packaging materials were used,and R. glutinosa were stored at three different temperatures and two kinds of confined environment.With verbascoside as evaluation index, and HPLC fingerprint technology was used to evaluate impact of different packaging materials, different storage on quality stability of R. glutinousa pieces. Result: On conditions of PTP aluminum foil,vacuum and refrigeration had the highest content of verbascoside,which were (0.028±0.011)%,(0.026±0.016)%,(0.025±0.014)%,respectively.The content of verbascoside in descending order as follows:PTP aluminum foil>polyethylene plastic bag>leather condensate film paper; vacuum>unvacuum; refrigerated>cool conditions>normal temperature.Optimum storage and curing condition was:PTP aluminum foil packaging,vacuum refrigeration. Conclusion: In this optimized condition,R. glutinousa pieces was stable,it could provide experimental basis for storage in actual production of R. glutinousa pieces.