乙肝病毒X基因与人肝细胞癌关系的研究

目的 探讨乙肝病毒（ＨＢＶ）Ｘ基因与肝癌发病的关系，为从分子水平上研究肝癌病因提供有关信息。方法 采用ＰＣＲ技术对ＨＢＶ不同状态的１０２例原发性肝细胞癌（ＰＨＣ）、３６例肝硬化（ＬＣ）及２８例慢性肝炎（ＣＨ）血清中ＨＢＶＸ基因进行了检测。结果 在ＨＢＶ５项标志均阴性的５３例ＰＨＣ检出Ｘ基因２０例（３７．７％），３１例ＬＣ中检出５例（１６．１％），１１例ＣＨ中检出２例（１８．２％）。所有ＨＢＶ Ｘ基

Study on Relation between Hepatitis B Virus X Gene and Primary Hepatocellular Carcinoma

Objective To approach the relation between HBV X gene and hepatocellular carcinoma in order to provide informations for etiological study of hepatic cancer in molecule levels. Methods The serums from 102 cases of primary hepatocellular carcinoma(PHC),36 cases of liver cirrhosis(LC) and 28 cases of chronic hepatitis(CH) were detected for the HBV X gene. Results 20 X gene positive were detected in 53 samples of PHC without HBV serological marker(37.7%),5 in 31 LC(16.6%) and 2 in 11 CH(18.2%). In another study on all the samples whose X gene were positive 20 S gene negative samples in 31 X gene positive PHC(64.5%), 5 in 8 LC(62.5%) and none in 11 CH were found. Conclusion HBV X gene integrated with hepatocellular chromosome was closely relative to development of LC and PHC; Integrated HBV X gene was closely related to deletion of p53 inhibiting cancer gene, and the integration of HBV X gene may be an important cause of hepatic cancer.