严重烧伤后大鼠心肌原癌基因c-fos、c-myc的表达

目的 研究严重烧伤后大鼠心肌细胞中c—fos、c—myc mRNA和蛋白的动态表达及意义。方法 复制wistar大鼠30％体表面积Ⅲ度烧伤模型，设烧伤组、补液组、维拉帕米治疗组和对照组，烧伤后在不同时相点处死动物取材。免疫组化方法检测c—fos、c—myc蛋白的表达，原位杂交法检测c—fos、c—myc mRNA的表达。结果 各实验组c—fos、c—myc蛋白及mRNA表达随伤后时间推移逐渐增强达到峰值后下降。组间比较，烧伤组、补液组、维拉帕米组c—fos、c—myc表达呈依次降低，差异有显性意义。结论 严重烧伤可诱导心肌内早期反应基因c—fos、c—myc的表达。不论是烧伤组、补液组还是维拉帕米组，c—fos、c—myc mRNA水平及蛋白水平都有不同程度的表达。其表达不同于心肌梗死和压力超负荷病理状态下基因表达特点，提示三问可能有着不同的信号转导机制和调节机制。

The significance of the postburn expression of proto-oncogenes c-fos and c-myc mRNA and proteins in rat myocardial cells

OBJECTIVE: To investigate the postburn expression of proto -- oncogenes c -- fos and c -- myc mRNA and proteins in rat myocardial cells following severe burn. METHODS: Wistar rats inflicted with 30% TBSA III degree burn on the back were employed as the model and randomly divided into four groups: burn (B), resuscitation (R), verapamil (V) and control (C) groups. The expressions of c -- fos and c -- myc mRNA were examined by in situ hybridization and that of the proteins by immunohistochemistry. RESULTS: The expressions of the proteins and mRNA of c -- fos and c -- myc in every experimental groups increased to peak values and then decreased along with time lapsing. And the expressions of c -- fos and c -- myc were weakest in B group, while that in V group was stronger than that in R group. CONCLUSION: The expression of postburn immediate -- early genes c -- fos and c -- myc could be induced in the myocardial cells in all the groups by severe burns. And the expression features were different from the gene expression in patients with myocardial infarction and with cardiac over -- load. This implied that there were different signal transduction and modulation mechanism among these pathological conditions.