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| 新生大鼠心肌细胞培养方法的改进 | |
| 引用本文： | 郭军,鲍翠玉,林国生,杨波. 新生大鼠心肌细胞培养方法的改进[J]. 心血管康复医学杂志, 2006, 15(6): 538-540 |
| 作者姓名： | 郭军 鲍翠玉 林国生 杨波 |
| 作者单位： | 1. 咸宁学院心血管疾病研究所,湖北,成宁市,437100;武汉大学人民医院心内科,湖北,武汉,430060 2. 咸宁学院心血管疾病研究所,湖北,成宁市,437100 3. 武汉大学人民医院心内科,湖北,武汉,430060 |
| 基金项目： | 湖北省教育厅资助项目(2004Q001) |
| 摘 要： | 目的:建立便捷,成熟的新生大鼠心肌细胞体外培养方法。方法:本实验对常用的培养方法加以改进,采用胰蛋白酶及Ⅰ型、Ⅱ型胶原酶共同消化法,分离新生大鼠心肌细胞,并对体外培养的心肌细胞的存活率、活力及纯度进行鉴定。结果:分离的心肌细胞存活率为96%,培养3 d后有60%~80%的细胞开始搏动,搏动频率平均50次/min,心肌细胞纯度在75%以上。结论:本实验建立的新生大鼠心肌细胞体外培养方法是成熟,可靠的。 |
| 关 键 词： | 心肌 大鼠 细胞培养 |
| 文章编号： | 1008-0074（2006）06-0538-03 |
| 收稿时间： | 2005-11-24 |
| 修稿时间： | 2005-11-24 |
Isolation and culture of cardiomyocytes in neonatal rat |
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| GUO Jun,BAO Cui-yu,LIN Guo-Sheng,YANG Bo. Isolation and culture of cardiomyocytes in neonatal rat[J]. Chinese Journal of Cardiovascular Rehabilitation Medicine, 2006, 15(6): 538-540 | |
| Authors: | GUO Jun BAO Cui-yu LIN Guo-Sheng YANG Bo |
| Abstract: | Objective:To establish a convenient and mature method of isolation and culture of cardiomyocytes in neonatal rat.Methods:Cardiomyocytes of neonatal rat were isolated in trypsin and collagenase.Then the livability,energy and the purity of cardiomyocytes were identified.Results:The livability of cardiomyocytes was 96%;60%~80% beating cardiomyocytes were observed and the beating frequency of cells was 50 beats/min after 3 days.The purity of cardiomyocytes was 75%.Conclusion:This method of isolation and culture of cardiomyocytes in neonatal rat was mature. |
| Keywords: | Myocardium Rat Cell cultural |
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