| 肺岩宁方对EMT间质细胞标志因子Vimentin、Fibronectin和N-cadherin表达的影响 |
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| 引用本文： | 赵晓珍,徐振晔,吴中华. 肺岩宁方对EMT间质细胞标志因子Vimentin、Fibronectin和N-cadherin表达的影响[J]. 上海中医药大学学报, 2008, 22(6): 47-49 |
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| 作者姓名： | 赵晓珍 徐振晔 吴中华 |
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| 作者单位： | 上海中医药大学附属龙华医院肿瘤二科,上海,200032;上海中医药大学科研实验中心,上海,201203 |
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| 基金项目： | 国家自然科学基金资助项目(30701114);;上海市重点学科建设资助项目(Y0302) |
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| 摘 要： | 目的：研究标志EMT过程发生的间质细胞因子Vimentin、Fibronectin和N—cadherin在C57小鼠Lewis肺癌中的表达，以及肺岩宁方对其mRNA表达的影响。方法：采用Real—TimePCR观察C57小鼠Lewis肺癌右腋下移植瘤和远处转移灶发生的肺组织中Vimentin、Fibronectin和N—cadherinmRNA表达及肺岩宁方对它表达的影响。结果：对于各组移植肿瘤组织中，间质细胞标志因子Vimentin经肺岩宁方治疗后，与模型组相比无差异（P〉0．05）；而Fibronectin和N—cadherin的表达经肺岩宁方治疗后，与模型组相比明显降低（P〈0．01）；对于各组肺组织中，Vimentin、Fibronectin和N—cadherin在转移率发生最高的模型组，其表达明显高于正常肺组织中的表达（P〈0．01），而经过肺岩宁方治疗后，与模型组相比，Fibronectin和N—cadherin标志因子表达均显著性下降（P〈0．05），而Vimentin标志因子治疗前后无差异（P〉0．05）。结论：EMT有可能参与了肺癌转移的发生；肺岩宁方具有下调标志EMT过程发生的间质细胞因子Fibronectin和N—cadherin的作用。
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| 关 键 词： | 肿瘤 上皮-间质细胞转化 基因表达 Vimentin Fibronectin N-cadherin |
Effects of "Feiyanning Decotion" on Expression of Mesenchymal Cell Markers Vimentin, Fibronectin and N-cadherin |
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| ZHAO Xiao-zhen,XU Zhen-ye,WU Zhong-hua. Effects of "Feiyanning Decotion" on Expression of Mesenchymal Cell Markers Vimentin, Fibronectin and N-cadherin[J]. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 2008, 22(6): 47-49 |
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| Authors: | ZHAO Xiao-zhen XU Zhen-ye WU Zhong-hua |
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| Affiliation: | ZHAO Xiao-zhen, XU Zhen-ye, WU Zhong-hua(1. Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine ;2.Scientific Experimental Center, Shanghai University of Traditional Chinese Medicine) |
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| Abstract: | Objective:To study the mRNA expression of mesenchymal cell factors Vimentin,Fibronectin and N-cadherin, markers of epithelial-mesenchymal transition(EMT) course in mice of C57 Lewis lung cancer, and effect of"Feiyanning Decotion"on them.Methods:The mRNA expression of Vimentin,Fibronectin and N-cadher in transplantation tumor tissue at right armpit and lung tissue of distant place metastasis, and effect of"Feiyanning Decotion"on them were detected with real-time PCR method.Results:In transplantation tumor ti... |
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| Keywords: | Vimentin Fibronectin N-cadherin |
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