马齿苋及其不同部位对高脂血清损伤脂肪细胞分泌TNF-α及IL-6的影响

目的:在体外探讨马齿苋不同部位含药血清对高脂血清损伤脂肪细胞分泌肿瘤坏死因子(TNF-α)及白介素-6(IL-6)的影响。方法:建立悬浮脂肪细胞模型,分为对照组及不同浓度载药血清组,以MTT比色法测定各组细胞存活率,以放射免疫法测定有效浓度组脂肪细胞分泌TNF-α及IL-6的浓度变化。结果:MTT试验结果表明,正常血清组细胞存活率明显高于高脂血清组(P<0.05)。与高脂血清组比,不同剂量高脂血清加载药血清组中,载药血清浓度为40%和20%的组别细胞存活率明显提高(P<0.05)。与正常血清组比,高脂血清组TNF-α及IL-6浓度明显增高(P<0.01)。与高脂血清组比较,除马齿苋低剂量20%组,其余各高脂血清加载药血清组均能明显降低TNF-α浓度(P<0.05或P<0.01);各剂量高脂血清加载药血清40%浓度组和马齿苋不饱和脂肪酸(MS)高剂量20%组均能明显降低IL-6浓度(P<0.05或P<0.01)。结论:马齿苋及其不同部位载药血清作用于高脂血清损伤的脂肪细胞,能显著提高脂肪细胞的存活率,并能降低脂肪细胞TNF-α及IL-6的分泌,这在一定程度上证实了马齿苋及其不同部位可以在体外细胞水平上改善脂代谢紊乱。

Effect of different parts of Portulace oleracea on the levels of TNF-α and IL-6 in the supernatant of cultured adipose cell

OBJECTIVE: To explore the effects of drug-carried serum of the different parts of Portulace olerace on cytokine TNF-alpha and IL-6 secreted by adipose cell in vitro. METHOD: Models of adipose cell were established by Rodbell method. Using the method of seropharmacology, the drug-carried serum of the different parts of P. olerace were prepared. The cell viability of each group was tested by. Methy thiazolyl tetrazolium (MTT) assay. The levels of TNF-alpha and IL-6 in the supernatant of cultured adipose cell were assayed by RIA. RESULT: MTT assay results showed the cell viability of normal serum group was significantly higher than that of high lipid serum( P < 0.05). Compared with the high lipid serum group, the cell viability of the drug-carried serum groups in 40% and 20% concentration were significantly increased( P < 0.05). The high lipid serum had a better effect on increasing the levels of TNF-alpha and IL-6 than the normal serum group (P < 0.01). Expect the drug-carried serum of P. olerace low dose group in 20% concentration, each drug-carried serum group could markedly lower the levels of TNF-alpha (P < 0.05 or P < 0.01). Each drug-carried serum group in 40% concentration and the drug-carried serum P. olerace MS high dose group in 20% concentration could markedly lower the levels of IL-6 (P < 0.05 or P < 0.01). CONCLUSION: The drug-carried serum of P. olerace and its different parts act on adipose cell damaged by the high lipid serum, significantly increas the cell viability in the groups in 40% and 20% concentration, and improve the disorder of lipid in differeut degrel by lowering the levels of TNF-alpha and IL-6 that adipose cell secreted in vitro.