肾透明细胞癌组织肾母细胞瘤过表达基因表达临床意义的探讨

目的：探讨肾母细胞瘤过表达基因（NOV）对肾透明细胞癌分级、分期、大小、增殖状态和预后的影响。方法：应用免疫组化法检测54例肾透明细胞癌组织NOV蛋白的表达，分析其与肾透明细胞癌分期、分级、大小、Ki-67指数的相关性，并比较NOV阳性组和阴性组的生存率差异。结果：NOV在肾脏透明细胞癌组织中的染色强度明显低于癌旁正常肾组织，22例（100％）癌旁正常肾组织均呈强阳性染色，54例肾癌组织中24例（44．4％）为弱阳性染色，8例（14．8％）为中等阳性染色；癌组织总阳性率（59．3％）亦显著低于癌旁正常肾组织（100％），P〈0．05。NOV阳性组的Ki-67指数（2．6±1．5）％，明显低于NOV阴性组的（4．2±2．1）％，P〈0．05。NOV表达与肿瘤分期及大小无相关性，P〉0．05。NOV阳性组术后5年特异生存率（82．6％）显著高于阴性组（50．3％），P〈0．05。结论：NOV对肾透明细胞癌起抑制作用，可作为预后判断的肿瘤标志。

Expression of NOV and its clinical significance in clear cell renal cell carcinoma

OBJECTIVE：To investigate the relationship between the expression of NOV and the stage, grade, proliferation index and prognosis of clear cell renal cell carcinoma（RCC）. METHODS： Fifty-four samples of clear cell RCC tissues were collected. NOV expression and Ki-67 index were examined by immunohistochemical staining. RESULTS： The immunostaining intensity of NOV was significantly lower in tumor compared to normal kidney tissue and strong NOV expressions were demonstrated in all 22 normal kidney tissues. In contrast only 24（44.4%） tumors were found to be weakly positive and 8（14.8%） tumors were found to be moderately positive. The positive rate of NOV was also lower in tumor （59.3%） compared to normal kidney tissue（100%）. The Ki-67 index was significantly higher in NOV negative tumor [（4.2 ± 2.1）% than that in NOV positive tumor （2.6 ± 1.5）%, P〈0.05]. NOV expression could not be correlated with stage,grade,and size of tumors. The NOV positive group showed significantly higher 5 year cancer specific survival rate compared to NOV negative group （82.6% vs 50.3% ,P〈0.05）. CONCLUSION： The results of this study suggest that NOV could play inhibitory role in clear cell RCC.