电针对子宫内膜异位症大鼠环氧合酶-2的影响

目的探讨电针对子宫内膜异位症(endometriosis,EMs)大鼠环氧合酶-2(cyclooxygenase-2,COX-2)的影响及其预防大鼠EMs的效应机制。方法建立大鼠EMs模型,随机分为模型组、电针组、COX-2抑制剂组、P450arom抑制剂组、去卵巢组,并设立假手术组。电针组电针血海、三阴交穴;COX-2抑制剂组采用塞莱西布胶囊生理盐水混悬液灌胃;P450arom抑制剂组采用阿纳曲唑片生理盐水混悬液灌胃;卵巢去势组切除双侧卵巢;假手术组和模型组捆绑固定并行生理盐水灌胃。治疗及对照处理2星期和4星期后处死各组大鼠,测量各组大鼠异位囊肿长、宽、高三径并计算平均值,取异位组织进行组织病理学观察,并测定血清及组织中COX-2的蛋白水平与组织中COX-2 mRNA的表达水平。结果异位囊肿三径平均值的比较,模型组均明显大于其他各组(均P<0.05),电针组明显小于模型组(P<0.05),且比较4星期与2星期电针组可见其生长较缓慢,异位内膜趋于萎缩,部分上皮细胞坏死;电针组血清中COX-2水平、异位内膜组织中COX-2水平及异位内膜组织中COX-2 mRNA表达水平均明显低于模型组(P<0.05),并随着疗程的延长效果更明显。结论电针可能通过调节异常升高的COX-2 mRNA水平,进而影响COX-2蛋白的合成,下调外周血和局部组织中COX-2的表达而预防大鼠EMs的发生与进展。

Effect of Electroacupuncture on Cyclooxygenase-2 in Endometriosis Rats

Objective To investigate the effect ofelectroacupuncture （EA） on cyclooxygenase-2 （COX-2） in endometriosis （EMs） rats and explore the mechanism of its action in preventing rat EMs. Method A rat EMs model was maded. The rats were randomly assigned to model, EA, COX-2 inhibitor, P450arom inhibitor, ovariectomy and sham operation groups. The EA group received electroacupuncture at points Xuehai（SP10） and Sanyinjiao（SP6）; the COX-2 inhibitor group, an oral gavage of a suspension of Celebrex in normal saline; the P450arom inhibitor group, an oral gavage of a suspension of Anastrozole in normal saline; the ovarieetomy group, surgical removal of both ovaries; the sham operation and model groups; binding fixation and an oral gavage of normal saline. All groups of rats were sacrificed after two and four weeks of treatment or control treatment. The length, width and height of the ectopic cyst were determined. An average value was calculated. The ectopic tissue was taken and examined histopathologically. The level of COX-2 in serum and the tissue and the expression of COX-2 mRNA in the tissue were measured. Result The average values of the three diameters of the ectopic cyst were significantly larger in the model group than in the other groups （all P〈0.05） and significantly smaller in the EA group than in the model group （P〈0.05）. A comparison between two and four weeks in the EA group showed that the ectopic cyst grew slowly, the ectopic inner membrane tended to atrophy and part of the epithelia became necrotic. The level of COX-2 in serum and the ectopic intimal tissue and the expression of COX-2 mRNA in the ectopic intimal tissue were significantly lower in the EA group than in the model group （P〈0.05）. The effects became more marked as the course of treatment lengthened. Conclusion Electroacupuncture may prevent the occurrence and development of EMs in rats by regulating abnormally high COX-2 mRNA levels, affecting the synthesis of COX-2 and downregulating the expression of COX-2 in peripheral blood and the local tissues.