肝癌病例中XRCC1基因多态性与环境因素的交互作用分析

目的：研究某些环境因素与X射线修复交叉互补组1（XRCC1）基因多态性间交互作用对肝细胞癌（HCC）发生危险性的影响。方法：采用单纯病例研究方法，调查500例HCC患者所接触的相关环境因素，用荧光实时定量PCR方法检测XRCC1—280、XRCC1—194和XRCC1—399单核苷酸多态性。结果：XRCC1—280与乙肝e抗体对HCC的发生有正相乘交互作用（P=0．047，OR=1．809，95％CI：1．007～3．249）；XRCC1—194与饮酒对HCC的发生有正相乘交互作用（P=0．041，OR=1．496，95％CI：1．016～2．204）；XRCC1—194与乙肝表面抗体阳性对HCC的发生有负相乘交互作用（校正后，P=0．045，OR=1．737，95％CI：1．013～2．979）。未发现XRCC1—399基因多态性与相关环境因素之间存在交互作用。结论：携带XRCC1—194突变等位基因与饮酒、乙肝表面抗体阳性对HCC可能存在交互作用；携带xRCC1—280突变等位基因与乙肝e抗体对HCC可能存在交互作用。

Interactions between XRCC1 gene polymorphism and specific environmental factors in HCC cases

OBJECTIVE： To explore the interactions between environmental exposure factors and genetic polymorphism in XRCC1 in HCC. METHODS： The research was designed by a case-only study based on hospitals. Studied cases were the 500 new HCC cases and their environmental exposed data were collected. TaqMan MGB Real-time quantitative polymerase chain reaction （RT-PCR） was used to detect single nucleotide polymorphism of XRCCl-194, XRCC1-280 and XRCC1-399. RESULTS： XRCC1-280 and Anti-Hbe had a positive multiplieative interaction on HCC occurrence （P= 0. 047, OR= 1. 809, 95 V00CI： 1. 007--3. 249）. XRCC1-194 and alcohol consumption had multiplicative interaction on HCC occurrence（P=0. 041, OR= 1. 496, 95%CI： 1. 016--2. 204）. XRCCl-194 and HBsAb had a negative multiplicative inter- action on HCC occurrence（P= 0. 045, OR： 1. 737, 95 % CI.. 1. 013-- 2. 979）. CONCLUSIONS： Carrying the XRCCl-194 mutant allele and drinking alcohol may have an interaction in HCC; carrying the XRCCl-194 mutant allele and HbsAb tend to an interaction related with HCC. Carrying the XRCC1-280 mutant allele and HbeAb are also likely to have an interaction in HCC.