具有不同转移潜力的卵巢癌细胞系基因表达谱的比较研究

目的:比较具有不同转移潜力的上皮性卵巢癌细胞系基因表达谱,探讨卵巢癌侵袭转移过程中的关键基因和基因群,为最终揭示卵巢癌侵袭转移过程的分子机制奠定基础。方法:采用人工基底膜侵袭实验比较SKOV3·ip1细胞与其母系SKOV3细胞的侵袭转移能力;用17000点人类基因组cDNA芯片杂交检测两细胞系基因表达谱的差异;用RT-PCR方法验证基因芯片结果。结果:与其母系SKOV3相比,SKOV3·ip1细胞侵袭转移能力更强;基因芯片检测到1557个2倍差异表达基因,其中包括nm23-H2,c-erbB-2等重要的侵袭转移基因及一些差异显著的未知基因和EST片段;nm23-H2基因RT-PCR结果验证了基因芯片实验结果。结论:上皮性卵巢癌细胞系SKOV3.ip1具有更强的侵袭转移能力;卵巢癌的侵袭转移是一个涉及nm23,c-erbB-2等重要基因和其他未知新基因等多个基因共同参与的复杂过程;基因芯片是高通量基因筛选的有利手段。

Analysis of gene expression patterns of ovarian cancer cell lines with different metastatic potential

Objective:To investigate the key genetic changes and molecular mechanism in the process of invasion and metastasis of human epithelial ovarian cancers.Methods:In vitro invasion assay was used to further testify that human epithelial ovarian cancer cell line SKOV3.ip1was more invasive and metastatic compared with its parental line SKOV3.17 000 human genome cDNA microarrays were used to compare the gene expression patterns of the two cell lines.RTPCR was performed to validate the results of the microarray.Results: Totally 1557 twofold differentially expressed genes were screened out by 1 human genome cDNA microarrays between the two cell lines,including some important genes such as,nm23H2,cerbB2 and other unknown genes or ESTs with remarkable fold changes.The results of the microarray experiment were further confirmed by RTPCR of nm23H2 gene.Conclusion: SKOV3.ip1 is more invasive and metastatic than its parental line.The invasion and metastasis mechanism of epithelial ovarian cancer is such a complex process that in which many important genes like nm23,cerbB2,as well as other unknown genes or ESTs are involved.Microarray is an effective way for highthroughout gene screening.