uPAR在鼻咽癌细胞系及其克隆株中的表达和作用

目的　探讨尿激酶型纤溶酶原激活剂受体 (uPAR )在鼻咽癌细胞系 (CNE 2Z)及其克隆株 (CNE 2Z H 5 ,CNE 2Z H 5 9)中的表达和作用。方法　采用逆转录聚合酶链反应 (RT PCR )方法 ,检测uPAR在基因转录水平的表达 ;采用侵袭抑制实验 ,观察uPAR在鼻咽癌细胞侵袭和转移过程中的作用。结果　uPAR在CNE 2Z H 5 9中表达最高 ,在CNE 2Z中表达最低 ,在CNE 2Z H 5中表达处于两者之间 ;抗uPAR抗体抑制CNE 2Z H 5 9的侵袭能力。结论　uPAR可能促进鼻咽癌细胞的侵袭和转移

Expression and the Effect of uPAR in Nasopharyngeal Carcinoma Cell Line and its Sub-lines

Objective To explore the expression and the effect of uPAR in Nasopharyngeal carcinoma cell line(CNE-2Z) and its sub-lines(CNE-2Z-H5,CNE-2Z-H5-9).Methods RT-PCR was used to detect the mRNA expression of uPAR.The experiments of invasive inhibition was used to observe the effect of uPAR on metastasis and invasion in NPC.Results mRNA expression of uPAR was the highest in CNE-2Z-H5-9 and the lowest in CNE-2Z.Anti-uPAR antibody inhibited the invasion of CNE-2Z-H5-9.Conclusion uPAR maybe promote the metastasis and invasion of NPC.