加贝酯对肝脏缺血再灌注损伤的保护作用

目的]探讨加贝酯对SD大鼠肝脏抗缺血再灌注损伤（HIRI）的效应及其可能的机制。方法]采用Pringle＇s法建立大鼠HIRI模型，将40只SD大鼠随机分成四组，每组10只：A组（假手术组）、B组（模型组）、C组（加贝酯处理组）、D组（盐水对照组）。分别测定血浆中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）浓度；测定肝组织中丙二醛（MDA）、超氧化物歧化酶（SOD）含量及光镜观察各组病理形态学变化。结果]B、C、D三组ALT、AST、MDA均显著高于A组（P〈0．05或P〈0．01），B、D两组又显著高于C组（P〈0．01）；A组的SOD含量明显地高于其他三组，而C组又明显地高于B、D两组（P〈0．01）。光镜下观察肝组织形态学变化，A组形态基本正常；B、D两组损伤最严重；而C组较B、D两组轻微。结论]肝脏缺血再灌注时，肝细胞功能受损，蛋白酶抑制剂加贝酯能明显减轻这种损伤，保护肝功能。

Effect of Gabexate Mesilate On Hepatic Ischemia Reperfusion Injury in Rat

Objective]To investigate the effect of gabexate mesilate（GM）on hepatic ischemia reperfusion injury in rat and its possible mechanism. Methods]Models of rats with hepatic ischemia reperfusion injury were established by Pringle＇s method. Forty SD rats were randomly divided into four groups （ n = 10 in each group）： group A （normal control group）, group B （model group）, group C （gabexate mesilate group） and group D （saline group）. In addition to histological examination of the liver, plasma alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, malondialdehyde （MDA）, superoxide dismutase（SOD） in liver tissue were also respectively measured in each group. Results] ALT, AST and MDA in Group B, C and D were all remarkably higher than those in Group A （ P %0.05 or P %0.01）, and those in Group B and D were remarkably higher than those in Group C （ P %0.01）. In addition, hepatic SOD activity of group A was significantly higher than that of the other groups, and that of group C was higher than that of group B and E（ P %0.01）. Histological changes in group A was almost normal, and those in group B and D were the most seri- ous, but those in group C were mild compared with those in group B and D. Conclusion] Liver function is damaged by hepatic ischemia reperfusion injury. GM, a protease inhibitor, can markedly alleviate this injury and protect liver function.