| 不同龋敏感人群变形链球菌分离株乳酸脱氢酶活性的初步研究 |
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| 引用本文： | 杨德琴,刘天佳,周学东,何奎芳,李颂,庄姮. 不同龋敏感人群变形链球菌分离株乳酸脱氢酶活性的初步研究[J]. 华西口腔医学杂志, 2005, 23(2): 116-118 |
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| 作者姓名： | 杨德琴 刘天佳 周学东 何奎芳 李颂 庄姮 |
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| 作者单位： | 1.口腔生物医学工程教育部重点实验室,四川大学,四川 成都610041;2.温州医学院 口腔内科教研室,浙江 温州325000;3.安徽医科大学口腔医院 牙体牙髓科,安徽 合肥230000;4.第四军医大学口腔医院 牙体牙髓科,陕西 西安710032 |
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| 基金项目： | 国家自然科学基金资助项目(30171013);贵州省自然科学基金资助项目[黔科合计(2004)3055] |
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| 摘 要： | 目的 初步探讨不同龋敏感人群变形链球菌乳酸脱氢酶(LDH)活性与龋病发生的关系,筛选不同LDH活力菌株。方法 从高龋和无龋个体分离变形链球菌各50株,应用经典考马斯亮蓝蛋白定量方法对菌细胞裂解液蛋白进行定量,通过还原性辅酶Ⅰ氧化法测定LDH活性,比较两组菌株LDH活性差异以及不同LDH活性的菌株在两组人群的分布。结果 两组菌株LDH的酶活性均值总体无差异(P>0·05);不同LDH活性的菌株在两组人群的分布不同(P<0·05)。结论 变形链球菌LDH活性与龋病的发生有一定相关性。
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| 关 键 词： | 变形链球菌 乳酸脱氢酶 蛋白定量 酶活性 |
| 文章编号： | 1000-1182(2005)02-0116-03 |
| 收稿时间： | 2005-04-25 |
| 修稿时间： | 2004-09-21 |
Study on Lactate Dehydrogenase Activity of Streptococcus mutans Isolates Derived from Caries-active and Caries-free Individuals |
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| YANG De-qin,LIU Tian-jia,ZHOU Xue-dong,HE Kui-fang,LI Song,ZHUANG Heng. Study on Lactate Dehydrogenase Activity of Streptococcus mutans Isolates Derived from Caries-active and Caries-free Individuals[J]. West China journal of stomatology, 2005, 23(2): 116-118 |
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| Authors: | YANG De-qin LIU Tian-jia ZHOU Xue-dong HE Kui-fang LI Song ZHUANG Heng |
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| Affiliation: | 1.KeyLab. ofO- ral Biomedical Engineering ofMinistry ofEducation, Sichuan University, Chengdu610041, China;2.Dept. ofOral Medicine, WenzhouMedical College,Wenzhou325000,China;3.Dept. ofOperative Dentistry andEndodontics, StomatologyCollege ofAnhui University ofMedicalSciences, Hefei230000, China;4.Dept. ofOperative Dentistry andEndodontics, The FourthMilitaryMedi- cal University, Xi′an710032,China |
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| Abstract: | OBJECTIVE: To preliminarily investigate the relationship between the Lactate dehydrogenase (LDH) activity of Streptococcus mutans (S. mutans) and dental caries initiation. METHODS: 100 S. mutans strains derived from caries-active and caries-free individuals were cultured in BHI medium supplemented with glucose. Cells were extracted and ruptured, and the extracted liquid protein was quantified with Coomassie brilliant blue G250 staining methods. LDH activity was assayed using the pyruvate-dependent oxidation of NADH-with and without FDP. RESULTS: LDH activity of the two groups strains had no difference (P > 0.05), but the distribution of differ class enzyme activity strains in the two groups was different (P < 0.05). CONCLUSION: LDH activity of S. mutans is correlated to the initiation of dental caries to some extent. The measurement methods in this study can be applied in preliminary quantitation of LDH activity and the screening of S. mutans strains. |
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| Keywords: | Streptococcusmutans lactatedehydrogenase proteinquantitation enzymeactivity |
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