鱼腥草注射液对噪声引起的小鼠学习记忆功能改变的作用

目的 研究噪声对小鼠学习记忆功能的影响,并探讨鱼腥草注射液对噪声引起的小鼠学习记忆功能改变的作用并分析其作用机制.方法 筛选小鼠随机分组,进行迷宫测试及脑内丙二醛(MDA)的含量和超氧化物歧化酶(SOD)、乙酰胆碱酯酶(AChE)、一氧化氮合酶(NOS)的活性的测定.结果 迷宫测试学习组中,噪声导致噪声组(12.1±1.20)次]较对照组(15.1±1.45)次]学习能力明显下降(P＜0.01),而鱼腥草注射液可改善噪声环境下小鼠的学习能力,噪声+药组(14.9±1.60)次]与噪声组比较正确率明显升高(P＜0.01).记忆组也出现噪声导致噪声组(5.20±1.40)次]较对照组(6.30±1.32)次]记忆能力明显下降(P＜0.01),而鱼腥草注射液可改善噪声环境下小鼠的记忆能力,噪声+药组(6.10±1.10)次]与噪声组比较正确率明显升高(P＜0.01).不管是学习组还是记忆组小鼠,噪声均导致小鼠脑内SOD和NOS的活性降低(P＜0.01),MDA含量和AChE的活性升高(P＜0.01);鱼腥草可改善噪声对小鼠脑内递质的影响,提高脑组织内SOD和NOS的活性(P＜0.01),降低脑组织中的MDA含量和AChE的活性(P＜0.01).结论 鱼腥草注射液对噪声引起的小鼠学习记忆功能减退具有预防作用,并具有抗氧化作用.

Effect of herba Houttuyniae injection on the change of learning and memory of mice in noises condition

Objective To study the effect of noise on the ability of learning and memory of mice,and observe the effect of herba Houttuyniae injection on the change of the ability of learning and memory in noise condition, and then analyses the mechanisms. Methods To choose suitable mice and divide into groups, applying Y-maze and colorimeter to determine the content of MDA and the enzyme activity of SOD, AChE and NOS of mice brain. Results Learning group in Y-maze, noise cause the ability of noise group (12.1±1.20) decrease(P＜0.01) compared with the control group(15.1±1.45), but herba Houttuyniae injection can reverse the ability of the mice, the correct frequency is much higher(P＜0.01)in herba Houttuyniae group(14.9±1.60). The results is same in the memory group, in Y-maze, noise cause the memory ability of noise group (5.20±1.40) decrease(P＜0.01) compared with the control group(6.30±1.32), but herba Houttuyniae injection can reverse the memory ability of the mice, the correct frequency is much higher(P＜0.01)in herba Houttuyniae group(6.10±1.10). Either learning or memory group mice, noise can decreased the activity of SOD and NOS of mice brain(P＜0.01), but the content of MDA and the ability of AChE elevated(P＜0.01). herba Houttuyniae injection can change the effect of noise to mice, the activity of SOD and NOS of mice brain elevated(P＜0.01) and the content of MDA and the ability of AChE decreased (P＜0.01). Conclusion herba Houttuyniae injection can prevent the ability of learning and memory of mice which decreased by noise, and have the effect of intoxication.