68例膀胱癌p53的检测及其预后意义

用抗p53单克隆抗体Do-7河检测68例膀胱移行上皮细胞癌．总阳性率44．1％16（30／68）。病理分级G_1p53阳性事28．6％（8／28）．G_248％（12／25），G_366．7％（10／15）（P＜0．05）。浸润型膀胱癌（T_2～T_4）的p53阳性率61．3％（19／31）．显著高于钱表型膀胱癌（Ta～T1）的29．7％（11／37）（P＜0．01）。随访材料（中位时间79个同）提示；p53阳性病例有很高的复发（P＜0．05〕、转移（P＜0．01）比例和较低的存活概率（P＜0．01）、本研究表明：膀恍癌p53过度表达与肿瘤恶性生物学行为密切相关，p53异常蛋白的检测可能是临床上有实用价值的客观预后指标。

Clinical Value of Nuclear Overexpression of p53 Protein in Transitional Cell Bladder Cancer

Using Do-7 monoclonal antibody, overexpression of p53 protein was studied by immunohistochemistry in paraffin-em-bedded tumour samples from 68 patients with urinary bladder TCC,and a total rate of 44. 1 % (30/68) acquaired. The present researchdernonatrated a significant difference of p53 positive fraction in grade category(Gl = 28. 6 %, G2: 48 M, G3: 66. 7 %, Pwto. o5 ),and espe-cially higher expression of invasive tumour(61. 3 % Vs 29. 1 % of supficia1 one, P<0. 01 ). An available foIlow up data with a mediumtime of 79 rnonths indicated,that positive p53 cases had rnore chance to recur or metastasize and had a rather low survivaI probability(P<0.01). It is our conclusion that overexpression of p53, to a certain extent, refIect the wicked biological behaviour of tumour,and p53imrn u noh istoc hemical expression st at u s may become a valuable prognostic marker for blad der cancer.