DNA-damaging potency and genotoxicity of aflation M1 in somatic cells in vivo of Drosophila melanogaster |
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| Authors: | Shibahara, Toshikazu Ogawa, H.Iyehara Ryo, Haruko Fujikawa, Kazuo |
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| Affiliation: | 1Drug Safety Research Center, Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd Tokushima Tokushima 771–01 2Department of Applied Chemistry, Faculty of Engineering, Kyushu Institute of Technology Tobata-ku Kitakyushu 804 3Department of Fundamental Radiology, Osaka University Medical School Suita, Osaka 565 4Atomic Energy Research Institute, Kinki University Higashiosaka, Osaka 577 Japan |
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| Abstract: | Aflatoxin M1 (AFM1), a metabolic hydroxylation product of aflatoxinB1 (AFB1), and the parent compound were comparatively assayedfor DNA-damaging potency and genotoxicity in vivo in Drosophilamelanogaster using, respectively, the mei-9a mei-41D5 DNA repairtest and the mwh/flr3 wing spot test. In the repair test, larvalstock, consisting of meiotic recombination-deficient doublemutant mei-9a mei-41D5 males and repair-proficient females,was exposed to the test agents, and the preferential killingof the mutant larvae was taken as evidence of the DNA-damagingeffect. In this test, AFM1 was registered as a DNA-damagingagent with an activity  3-fold lower than that of AFB1. In thewing spot test, where larval flies, trans-heterozygous for thesomatic cell markers mwh and flr3, were treated and the wingswere inspected at adulthood for spots manifesting the phenotypesof the markers, AFM1 exerted a genotoxic effect compatible tothat of AFB1. Based on these results and other data, we predictthat AFM1 may be genotoxic in mammalian in-vivo systems as well. 5To whom correspondence should be addressed |
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