| 恙虫病立克次体sta56基因片段的克隆 |
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| 引用本文： | 陈添胜. 恙虫病立克次体sta56基因片段的克隆[J]. 中国人兽共患病杂志, 1998, 14(5): 69-70 |
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| 作者姓名： | 陈添胜 |
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| 作者单位： | 广东省流行病防治研究所!广州，510300(陈添胜，谭丽霞，林普生，罗会明)，广东省口腔医院(许曼波)，中山医科大学(黎家灿，冯慧敏) |
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| 摘 要： | 应用半套式PCR技术从Karp株恙虫病立克次体基因组中扩增出872hpDNA片段。该片段经Pstl消化后，与经PStl酶解的pUC18连接，转化大肠杆菌，电泳初筛得pUCRL3等重组质粒。pUCRL3经PCR及酶切证实含约294hp恙虫病立克次体sta56基因片段，可作为检测恙虫病立克次休的特异核酸探针。
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| 关 键 词： | 恙虫病立克次体 分子克隆 sta56基因 |
LONING OF PARTIAL STA56 GENE OF RICKETTSAI TSUTSUGAMUSHI |
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| Chen Tiansheng,Tan Lixia,Lin Pusheng et al. LONING OF PARTIAL STA56 GENE OF RICKETTSAI TSUTSUGAMUSHI[J]. Chinese Journal of Zoonoses, 1998, 14(5): 69-70 |
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| Authors: | Chen Tiansheng Tan Lixia Lin Pusheng et al |
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| Abstract: | From the genome of Rickettsia tsutsugamushi, strain Karp,an 872 --hp fragment of partial sta56 gene was amplified by semi--nested polymerase chain reaction. A 294--hp Pstl fragment deriving from the 872hp one was cloned into pUC18 yielding pUCRL3,the recombinant plasmid was confirmed by PCR using. R.tsutsugamushi specific primer and pUC/M13 forward sequencing primer. The probe can be used to detect. R.tsul-sugamushi DNA by nucleic acid Hybridization. |
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| Keywords: | Rickettsia tsutsugamushi Molecular cloning sta56 gene |
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