溴环己胺醇对通气机导致肺损伤保护作用的研究

目的 探讨抗氧化剂溴环己胺醇对通气机导致肺损伤（ＶＩＬＩ）的保护作用。方法 将３０只健康大鼠随机分为３组,Ａ组为对照组（常规机械通气）,Ｂ组为大潮气量通气组,Ｃ组为大潮气量加溴环己胺醇干预组,分别检测ＶＩＬＩ大鼠肺灌洗液（ＢＡＬＦ）中蛋白质、白细胞、肿瘤坏死因子α（ＴＮＦ－α）、白细胞介素１β（ＩＬ－１β）、白细胞介素８（ＩＬ－８）以及肺匀浆中丙二醛（ＤＭＡ）、超氧化物歧化酶（ＳＯＤ）、蛋白含量均

Prophylaxis against ventilator-induced lung injury by Ambroxol

OBJECTIVE: To investigate the protective effect of Ambroxol on ventilator induced lung injury (VILI) of rats. METHODS: 30 healthy SD rats were randomly divided into three groups: group A was served as control group (n = 10, V(T) 8 ml/kg), group B received large V(T) ventilation (n = 10, V(T) 40 ml/kg), group C received large V(T) ventilation with pre-treatment of IP ambroxol. The contents of protein, TNF-alpha, IL1-beta, IL-8 in BALF and MDA, SOD, GSH levels in the homogenate of the rat lungs were assayed respectively. Total white blood cells in BALF were counted; The severities of lung injury of the three groups were assessed under a light microscope. RESULTS: The albumin and total protein contents in BALF were significantly higher group A than in groups B and C. A large quantity of WBCs infiltration were found in BALF of group B, while the amount of WBCs were decreased in group C. TNF-alpha, IL-1beta and IL-8 level in BALF of group B were 101.6 pmol.L(-1).mg(-1) Pr +/- 37.4 pmol.L(-1).mg(-1) Pr, 0.47 microgram.L(-1).mg(-1) Pr +/- 0.14 microgram.L(-1).mg(-1) Pr and 2.02 microgram.L(-1).mg(-1) Pr +/- 0.49 microgram.L(-1).mg(-1) Pr, respectively. MDA, one of the parameters reflecting the extent of oxidative reaction, were produced profoundly in the lungs of group B, but the local levels of SOD, which reflects the ability of antioxidation, were decreased significantly in group B. There existed significant differences of the above parameters between group B and group A. Ambroxol could significantly decrease the locally produced TNF-alpha and inhibit the oxidative reactions, and increase the locally antioxidative potency. Severe acute lung injuries were detected in group A by histological examination and the extent of injury was much lighter in group C than in group B. CONCLUSION: Locally increased inflammatory cytokines formation and the imbalanced oxidative/antioxidative reaction play important roles in the pathogenesis of VILI. Ambroxol has an obvious protective effect on VILI through its antioxidant/anti-inflammation potent.