茶多酚对H_2O_2诱导HL-60细胞癌基因表达的影响

目的　观察茶多酚对 H2 O2 诱导 HL- 60细胞癌基因表达的影响 ,同时观察细胞 DNA片段化程度的变化。方法　用二苯胺法对小片段 DNA进行分析 ,流式细胞仪观察癌基因表达变化。结果　 H2 O2 打断 DNA的能力与其作用浓度及时间有关 ,H2 O2 可使细胞 c- fos,c- jun,c- myc,bcl- 2 ,p53表达发生变化 ,茶多酚可以影响 H2 O2 诱导的 DNA损伤及癌基因表达。结论　茶多酚在一定浓度下 ,可以抑制 H2 O2 诱导的 DNA氧化损伤 ,并抑制癌基因表达的变化。高浓度茶多酚可以增强 H2 O2 的效应

Effect of teapolyphenol on oncogenes expression in HL-60 cell treated with H_2O_2

? Objective To study the effect of the teapolyphenol on oncogenes expression and the change of fragmentation of DNA in HL 60 cells that damaged by H 2O 2.Methods Fragmentation of DNA was detected by diphenylamine.Oncogenes expression was detected by flow cytometry.Results Fragmentation of DNA was increased and some oncogenes expression,including c fos?c jun?c myc?bcl 2?p53 changed in cells treated with different concentratins of H 2O 2,while teapolyphenol can affect fragmentation of DNA and some oncogenes expression induced by H 2O 2.Conclusions The effect of tepolyphenol on fragmentation of DNA and some oncogenes expression depended on the concentrations of teapolyphenol,the lower concentrations of teapolyphenol inhibited the oxidization of H 2O 2?