土知母药材中射干苷定性与定量分析研究

目的：建立土知母药材中射干苷定性与定量分析方法。方法：采用TLC法对土知母药材中射干苷进行定性鉴别；HPLC法对土知母药材中射干苷进行含量测定。色谱柱：Diamonsil C18（200 mm x4.6 mm，5μm），流动相：乙睛-0.2%磷酸溶液（20：80），流速：1.0 mL·min-1，波长：265 nm，柱温：25益。结果：土知母药材定性方法专属性强，无其它成分干扰；射干苷在0.12-2.22μg范围内与峰面积响应值呈良好的线性关系，平均加样回收率为100.96%。结论：本法简便可行，重复性好，可用于土知母药材的质量控制。

Quality and Quantity Analysis on Tectoridin of Iris Tectorum Maxim

This study was aimed to establish the quality and quantity analysis methods of tectoridin contented in the Iris tectorum Maxim. Thin layer chromatography （TLC） method was used to give quality analysis on tectoridin in I. tectorum. And the HPLC method was used to give quantity analysis on tectoridin in I. tectorum. The Diamonsil C18 （200 mm ＆#237; 4.6 mm, 5 μm） column was used as analytical column. The acetonitrile-0.2% phosphoric acid （20：80） was used as mobile phase at the flow rate of 1.0 mL＆#183;min-1. The detection wavelength was 265 nm and the column temperature was 25℃. The results showed that the quality analysis of I. Tectorum had specific identification with-out the interference of other ingredients. The amount of inlet tectoridin had a good linearity with the response val-ue of peak area in the range of 0.12-2.22 μg. The average recovery rate was 100.96%. It was concluded that this method was simple and reproducible, which can be used in the quality control of I. tectorum.