黄芪的含药血清诱导K562细胞表达^Gγ-mRNA和合成HbF

目的研究黄芪对K562细胞的^Gγ-珠蛋白基因表达的作用。方法以K562细胞为体外模型，用联苯胺染色方法来确定黄芪的含药血清诱导K562细胞向红系分化的作用；用RT—PCR方法研究黄芪的含药血清诱导K562细胞^Gγ-珠蛋白mRNA的表达；用Western Blot方法研究黄芪的含药血清诱导K562细胞HbF的合成。结果黄芪的含药血清作用于K562细胞5d后联苯胺染色阳性率达19．8％；同时伴有^Gγ-珠蛋白mRNA的表达的增加（最高可达3．6倍）和HbF的合成的增加，与丁酸钠组比较，黄芪组诱导K562细胞^Gγ-珠蛋白基因mRNA的表达可以持续3～5d，而丁酸钠组只有1d。结论黄芪有望成为一种有效的γ蛋白基因诱导剂。

Accumulation of Gγ-globin mRNA and Induction of HbF in K562 Cells Induced by Serum Contained Huangqi

Aim To study the effect of accumulation of ^Gγ-globin gene mRNA expression and induction of HbF in K562 cells induced by Huangqi in vitro. Methods Benzidine staining was used to confirm the effect of Huangqi on hemoglobin synthesis in K562 cells. RT-PCR was applied to study ^Gγ-globin gene mRNA expression. Western blotting technique was applied to study the level of HbF in K562 cells. Results After 5 days of Huangqi medium stimulation, the positive rate of Benzidine staining（ BZ% ） in K562 cells was 19.8% ;the level of ^Gγ-mRNA was increased （3.6 fold ） ;the level of of HbF was also increased. Compared to the butyrate - induced group, ^Gγ-mRNA expression in Huangqi medium-induced K562 cells lasted 3 to 5 days, but in the K562 cells induced by butyrate, it lasted only one day. Conclusion Huangqi would be a promising inducer of γ-globin gene expression.