急性胰腺炎大鼠胰腺细胞凋亡与胰腺炎症细胞的关系

目的: 探讨急性胰腺炎(AP)胰腺细胞凋亡的可能机制.方法: 胆胰管逆行加压注射4%的牛磺胆酸钠诱导大鼠AP,建立模型,应用末端脱氧核苷酸转换酶(TdT)介导的原位末端标记(TUNEL)法检测术后3h、6 h和12 h胰腺细胞凋亡情况,并对胰腺炎症细胞进行分类记教.结果: 胰腺炎组术后6 h胰腺凋亡细胞显著高于3 h和12 h(3 h P<0.05,12 h P<0.01);白细胞进行性增多,6 h巨噬细胞百分比明显高于3 h和12h(3 h P<0.05,12 h P<0.01),中性粒细胞百分比显著低于3 h和12 h(3 h P<0.05,12 h P<0.01).结论: AP胰腺细胞凋亡可能与炎症细胞的不同种类有关.

Study on the relationship between apoptosis and leukocytes in rats with acute pancreatitis

Objective:To investigate the mechanisms of pancreatic acina cell apoptosis in rats with acute pancreatitis(AP).Methods:The model of AP was induced by retrograde injection of 4% sodium taurocholate into biliopancreatic duct in rats. A terminal deoxynucleotidyl transferase mediated dUTP biotion nick end labeling(TUNEL) was used to detect apoptosis. Leukocytes were identified by morphological features on paraffin sections obtained from the pancreas stained with HE.Results:Apoptosis index of AP rats at 6 h was higher significantly than that at 3,12 h(3 h P<0.05,12 h P<0.01,respectively). The types of leukocyte differed significantly in AP rats. Compared with that at 3,12 h,the percentage of macrophages at 6 h were significantly higher(3 h P<0.05,12 h P<0.01,respectively),while the percentage of neutrophils at 6 h were significantly lower(3 h P<0.05,12 h P<0.01,respectively).Conclusion:Pancreatic acina cells undergoing apoptosis may result from differences in inflammatory cell infiltration in AP.