辛伐他汀对高糖诱导的肾小管上皮细胞转分化的影响

目的:观察辛伐他汀对高糖诱导的肾小管上皮细胞转分化的作用。方法:大鼠肾小管上皮细胞随机分成三组(n=8):对照组,高糖组和辛伐他汀组。对照组加培养液,高糖和辛伐他汀组在培养液中加葡萄糖,终浓度为30mmol/L,辛伐他汀组加入终浓度为1μmol/L辛伐他汀。在6,12,24,48,72 h时用免疫细胞化学和Western blot检测α-SMA的表达。结果:对照组各时间段均无α-SMA表达。高糖组6 h时出现α-SMA阳性表达,48 h达高峰。与高糖组比较,辛伐他汀组各时间点α-SMA的表达均明显减少(P<0.05)。结论:辛伐他汀可抑制高糖诱导的大鼠肾小管上皮细胞转分化。

Effects of Simvastatin on the Transdifferentiation of Renal Tubular Epithelial Cells Induced with High Glucose

Objective To investigate the effects of simvastatin in renal tubular epithelial cells transdifferentiation induced with high glucose.Methods Cultured renal tubular epithelial cells were randomly divided into three groups（ n = 8 ） ：control group （group C ） , high glucose group （group D） and simvastatin group（ group S） , and given culture solution, high glucose （terminal concentration ：30 mmoL/L）, high glucose （ terminal concentration ： 30 mmoL/L） plus simvastatin （ 1 μmoL/L）, respectively. The expression of α-SMA was detected with immuocytochemistry and Western blot at 6,12,24,48 and 72 hours, respectively. Results The expression of α-SMA was not detected at all the time points. The positive expression of α-SMA in group D was detected at 6 h and reached peak at 48 h. The expression of α-SMA in group S was significantly lower than that of group D at all the time points（ P 〈 0.05）. Conclusion Simvastatin could inhibit the transdifferentiation of renal tubular epithelial cells induced with high glucose.