Bacterial LPS Mediated Acute Inflammation-induced Spermatogenic Failure in Rats: Role of Stress Response Proteins and Mitochondrial Dysfunction |
| |
Authors: | Mallikarjuna Reddy Metukuri Chandra Mohan T Reddy P R K Reddy Pallu Reddanna |
| |
Institution: | (1) Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, AP, India, 500 046;(2) Department of Medicine, E1114 Biomedical Sciences Tower, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA USA, 15213; |
| |
Abstract: | Bacterial Lipopolysaccharide (LPS) induced inflammation is implicated in the infection associated testicular tissue damage.
Earlier, using a LPS induced acute endotoxemic rat model, we have shown the involvement of inflammation-induced oxidative
stress in the impaired steroidogenesis and spermatogenesis. In the present study, we report a significant induction (more
than 2-fold) of stress response proteins HSP-60, HMGB-1 and 2 in the testes, as early as 6 h after LPS injection with a later
decrease. This induction of acute stress is closely followed by a significant reduction (74%) in Bcl2/Bax ratio along with
leakage of cytochrome c (3 fold increase, p < 0.05) from mitochondria and increased caspase-3 activity levels (2.9 fold, p < 0.05) at 12 h and 24 h post LPS injection respectively. Further studies on PARP cleavage revealed a pattern similar to
necrotic death during early periods (3 h to 24 h) and apoptosis at later periods (24 h to 72 h) after LPS treatment. In conclusion,
the present study shows the involvement of stress response proteins and mitochondrial dysfunction in LPS-induced germ cell
death in male rats. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|