肝硬化大鼠肝部分切除术后肝再生的干预研究

目的　以肝硬化大鼠为动物模型 ,研究药物对肝硬化大鼠肝部分切除术后肝再生的影响。方法　取健康的Wistar雄性大鼠 6 4只 ,以 6 0 ?l4油溶液 0 .3ml/ 10 0 g皮下注射 ,同时饮用 5 %酒精溶液 ,4 5d后制成肝硬化动物模型。模型大鼠随机分为 4组 ,16只 /组。全麻下均行左、中叶肝切除术。术后各组按以下方案处理 :A组 (对照组 )注射生理盐水 1mg/ (kg·d) ,B组为泮托拉唑组 ,注射 0 .2mg/ (kg·d) ,C组为重组人生长激素组 ,注射 0 .5U/ (kg·d) ,D组为两药合用组 ,同时给予泮托拉唑注射 0 .2mg/ (kg·d) ,重组人生长激素注射 0 .5U/ (kg·d) ) ,连续给药 1周。抽取静脉血样 ,取肝脏组织 ,检测肝功能、有丝分裂指数 (MI)、增殖细胞核抗原 (PCNA)、细胞核DNA含量。结果　泮托拉唑组、重组人生长激素组、两药合用组MI、PCNA阳性染色细胞量、细胞核DNA含量均高于对照组 (P <0 .0 5 ) ,两药合用组MI、PCNA阳性染色细胞量、细胞核DNA含量均高于泮托拉唑组、重组人生长激素组 (P <0 .0 5 ) ,但各组间肝功能变化无明显差异。结论　泮托拉唑组及重组人生长激素均对肝硬化大鼠肝部分切除术后肝细胞再生有促进作用 ,两药联合应用肝细胞再生更明显 ,其详细机制须待进一步研究。

Hepatocyte regeneration and effects of some medicines after partial hepatectomy in rats with liver cirrhosis

Objective To study the regenerative capability of liver after partial hepatectomy and the effects of some medicines in the hepatic regeneration in cirrhotic rats. Methods Liver cirrhosis was induced by the injection of 60% CCl 4 0.3 ml/100 g body weight each time. At the same time, all rats received 5% alcohol solution as the only water source.After 45 days, cirrhosis models were eastablished. Cirrhotic rats were randomly separated into four groups(n=16),and each rat underwent 70% hepatectomy. The drugs were administrated on the operative day and lasted for one week. Group A was given 0.9% NS sc.1 mg/kg as control; group B pantoprazole 0.2 mg/kg, sc.daily; group C, rhGH 0.5 U/kg ,sc.daily;group D,pantoprazole 0.2 mg/kg and rhGH 0.5 U/kg,sc.daily. One week after treatment. Rats were killed, and blood was taken from the inferior vena cava for the determination of ALT and AST. The liver specimens were examined with a light microscope MI(mitotic index) ,PCNA(proliferative cell nuclear antigen) and contents of DNA in cell nuclear were determined. Results MI, PCNA and contents of DNA in cell nuclear in pantoprazole , rhGH and the combination with pantoprazole plus rhGH groups were significantly higher than in control (P<0.05). MI, PCNA and the contents of DNA in cell nuclear in pantoprazole plus rhGH groups was higher than those of pantoprazole , rhGH groups. But there was no difference in liver function alternation. Conclusion Results suggests that rhGH ,pantoprazole and pantoprazole plus rhGH can enhance the cirrhotic liver regeneration after hepatectomy.Combination of pantoprazole and rhGH could enhance more liver cell regeneration than the others.