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| 反义寡核苷酸降低细胞株SMMC—7721／ADM之MRP基因表达的研究 | |
| 引用本文： | 杨家印,罗华友,林琦远,严律南,林萍,雷松,黄代新,赵永恒.反义寡核苷酸降低细胞株SMMC—7721／ADM之MRP基因表达的研究[J].中国普外基础与临床杂志,2001,8(3):141-144. |
| 作者姓名： | 杨家印 罗华友 林琦远 严律南 林萍 雷松 黄代新 赵永恒 |
| 作者单位： | 1. 华西医科大学附属第一医院普外科 2. 华西医科大学附属第一医院肿瘤研究所 3. 华西医科大学附属第一医院生物治疗中心 4. 华西医科大学法医学院物证室 |
| 基金项目： | 国家自然科学基金资助(批准号：39770723) |
| 摘 要： | 目的 研究反义硫代磷酸寡核苷酸（AS－ODN）抑制人肝癌细胞耐药株（SMMC－7721／ADM）MRP基因表达的作用。方法 用人工合成互补于MRP基因mRNA特定片断的反义硫代磷酸寡核苷酸，以脂质体Lipofectamine为载体转染SMMC－7721／ADM细胞株，用RT－PCR测定mRNA表达，流式细胞技术测定细胞膜MRP1蛋白P190表达及MTTI地检测细胞对柔红霉素（DNR）和阿霉素（ADM）的敏感性。结果 AS－ODN能抑制AMR mRNA和其蛋白P190表达，提高细胞对DNR和ADM的敏感性。结论 （1）AS－ODN能降低MRP基因表达;(2)MRP介导的多药耐药（MDR）是SMMC－7721／ADM耐药的重要机理之一。 |
| 关 键 词： | 反义硫代磷酸寡核苷酸 多药耐药性 人肝癌细胞 MRP 基因表达 |
| 文章编号： | 1007-9424(2001)03-0141-04 |
| 修稿时间： | 2000年11月24 |
EFFECTS OF PHOSPHOROTHIOATE ANTISENSE OLIGONUCLEOTIDES TARGETING MRP mRNA ON DRUG-RESISTANT CELL LINE SMMC-7721 |
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| YANG Jia-yin,LUO Hua-you,LIN Qi-yuan,et al..EFFECTS OF PHOSPHOROTHIOATE ANTISENSE OLIGONUCLEOTIDES TARGETING MRP mRNA ON DRUG-RESISTANT CELL LINE SMMC-7721[J].Chinese Journal of Bases and Clinics In General Surgery,2001,8(3):141-144. | |
| Authors: | YANG Jia-yin LUO Hua-you LIN Qi-yuan |
| Institution: | YANG Jia-yin,LUO Hua-you,LIN Qi-yuan,et al. Department of General Surgery,First Affiliated Hospital,West China University of Medical Sciences,Chengdu 610041 |
| Abstract: | Objective To investigate the effect of phosphorothioate antisense oligonncleotides (AS-ODN) on sup pressing multidrug resistance-associated protein gene(MRP) in human drug-resistant hepatocellular carcinoma cell line (SMMC-7721/ADM). Methods Cell line was transfected with a synthetic S-ODN complementary to the coding region of MRP mRNA, Lipofectamine acting as carrier. The drug sensitivity was measured by MTT assay. The expression of MRP mRNA was detected by RT-PCR and the expression of P190, was detected by flow cytometry. Results AS-ODN inhibited expression of MRP mRNA and P190 and promoted sensitivity to daunorubicinum and adriamycinum. Conclusion AS-ODN can reduce the expression of MRP gene. MDR caused by MRP is an important cause of multidrug resistance of SMMC-7721/ADM. |
| Keywords: | Phosphorothioate antisense oligonucleotides Multidrug resistance Human hepatocellular carcinoma cell |
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