Specific detection of avian pneumovirus (APV) US isolates by RT-PCR |
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| Authors: | H J Shin G Rajashekara F F Jirjis D P Shaw S M Goyal D A Halvorson K V Nagaraja |
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| Institution: | (1) Department of Veterinary PathoBiology, University of Minnesota, St. Paul, Minnesota, U.S.A., US;(2) Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota, U.S.A., US |
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| Abstract: | Summary. This report details the development of an RT-PCR assay for the specific detection of US isolates of avian pneumovirus (APV).
Of the several primer pairs tested, two sets of primers derived from the matrix gene of APV were able to specifically detect
the viral RNA of APV. The nucleotide sequence comparison of the PCR products of APV isolates from Minnesota suggested that
these viruses were closely related to the Colorado strain of APV, but were distinct from subtypes A and B European isolates
of turkey APV (turkey rhinotracheitis: TRT). This M gene-based PCR was found to be very specific and sensitive. APV as low
as 8 × 10−5 TCID50 (0.0323 μg/ml) could be detected using this assay. In addition, the two primers were able to differentiate isolates from
turkeys in Minnesota.
Received June 15, 1999 Accepted January 3, 2000 |
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