hCAP-18/LL-37基因转染对Lewis肺癌细胞增殖及凋亡的影响

目的:观察人源抗菌肽hCAP-18/LL-37基因转染对Lewis肺癌细胞增殖的抑制作用，探讨其抗肿瘤的可能机制。方法:将含hCAP-18/LL-37的真核表达质粒瞬时转染Lewis肺癌细胞,同时设正常对照组、转染空载体组和转染重组基因组，MTT比色检测Lewis肺癌细胞转染后48和72 h的增殖活性，流式细胞术和荧光染色检测细胞凋亡。免疫细胞化学染色检测Bax、Bcl-2蛋白表达。结果：转染重组基因组Lewis细胞增殖受到明显抑制，与转染空载体组比较，转染后48和72 h的抑制率分别为3.51%和17.65%；流式细胞术检测转染重组基因组凋亡率为7.4%，正常对照组凋亡率为4.4%，转染空载体组凋亡率为5.2%；免疫细胞化学染色，转染重
组基因组Lewis细胞Bax蛋白表达增加，与对照组比较差异有显著性(P<0.05)；Bcl-2蛋白表达减少，但与对照组比较差异无显著性(P<0.05)。结论：hCAP-18/LL-37基因瞬时转染Le
wis肺癌细胞，可抑制肿瘤细胞生长、调控凋亡相关基因Bax、 Bcl-2表达，诱导肿瘤细胞凋亡。

Effect of hCAP-18/LL-37 gene transfection on proliferation and apoptosis of Lewis cells

Objective To investigate the inhibitory effect of hCAP-18/LL-37 gene transfection on proliferation in Lewis cells,and to study the possible antitumor mechanism.Methods DNA of hCAP-18/LL-37 transient transfection mediated by Polyfect was employed.The experimental cells were classified into untransfected cells,pcDNA4/Myc-His transfected cells and pcDNA4/Myc-His-LL-37 transfected cells.The proliferating abilities of Lewis cells 48 and 72h after transfection were measured by MTT colorimetric assay.The apoptosis...