| AMOS-PCR对布鲁氏菌种型鉴定的应用 |
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| 引用本文： | 姜海,崔步云,赵鸿雁,朴东日,李兰玉.AMOS-PCR对布鲁氏菌种型鉴定的应用[J].中国人兽共患病杂志,2009,25(2):107-109. |
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| 作者姓名： | 姜海 崔步云 赵鸿雁 朴东日 李兰玉 |
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| 作者单位： | 中国疾病预防控制中心传染病预防控制所; |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的评价AMOS-PCR方法鉴定布鲁氏菌种型的特异性和实用性。方法用已建立的AMOS-PCR方法在国内检测标准菌株和对国内分离的布鲁氏菌地方株种型进行检测。结果羊种布鲁氏菌、牛种布鲁氏菌1,2,4、猪种布鲁氏菌1型和绵羊附睾布鲁氏菌有特异条带。结论AMOS-PCR是一种快速、简便、特异的鉴定布鲁氏菌的方法之一。
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| 关 键 词： | 布鲁氏菌 AMOS-PCR 鉴定 |
| 收稿时间： | 2009-02-20 |
Use of AMOS-PCR assay for the species identification of Brucella |
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| JIANG Hai,CUI Bu-yun,ZHAO Hong-yan,PIAO Dong-ri,LI Lan-yu.Use of AMOS-PCR assay for the species identification of Brucella[J].Chinese Journal of Zoonoses,2009,25(2):107-109. |
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| Authors: | JIANG Hai CUI Bu-yun ZHAO Hong-yan PIAO Dong-ri LI Lan-yu |
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| Institution: | (National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206,China) |
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| Abstract: | To evaluate a rapid and specific PCR-based diagnostic method for the species identification of Brucella, the primers used in PCR assay were designed according to the Brucella insertion sequence IS711 and its neighbouring chromatid DNA sequence in order to establish the abbreviated Brucella AMOS- PCR assay. By using this method, it can identify and differentiate B. abortus biovars 1,2 and 4： B. melitensis; B. suis biovar 1 and B. ovis. and it is evident that AMOS assay appears to be a rapid , simple diagnostic and specific method. |
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| Keywords: | AMOS-PCR |
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