酩蛋白分解显色法测定血浆纤溶抑制活性和纤溶激活活性

报告了用酪蛋白分解显色法测定血浆纤溶抑制活性(FIA)和纤溶激活活性(FAA)的方法。原理是样本中产生的纤溶酶作用于酪蛋白,生成可溶性酪蛋白片段,加入Folin酚试剂后显色,在620nm波长处测光吸收度,以反映样本中FIA或FAA。两种活性测值可互相比较,计算比值。

DETERMINATION OF PLASMA FIBRINOLYTIC INHIBITION ACTIVITY AND FIBRINOLYTIC ACTIVATION ACTIVITY WITH A CASEINOLYTIC METHOD

A caseinolytic method for determination of plasma fibrinolytic inhibition activity(FIA)and fibinolytic activation activity(FAA)was described.1.Determination of FIA:urokinase was selected as plasminogen activator and added to tested plasma to convert the zymogen to its active form of plasmin,In the process of the convertion the plasmin inhibitors and the plasminogen activator inhibitors exerted their inhibitory effects on the formation of plasmin in plasma.The finally formed plasmin was negatively correlated to the fibrinolytic inhibition capability in tested plasma.2.Determination of FAA:since the FIA was always predominant over the FAA in plasma the action of inhibitory factors should be eliminated before plasma FAA was to be measuered.The inactivation of the inhibitory factors was achieved either by addition of sodium dodecyl sulfate(SDS)to plasma or by acidification of plasma.The function of activation activity was then recovered by subsequent dilution of plasma.To the diluted plasma added was fixed amount of purified plasminogen which was converted to plasmin by FAA in plasma.The final formation of plasmin was positively correlated to FAA in plasma.The design of the assay was based upon the proteolytic action of plasmin on casein to produce soluable casein fragments which could be electrophotometrically measured at 620 nm by addition of Folin phenol reagent,The final chromometry as so masured reflected FIA or FAA in plasma,Differrent concentrations of standard tyrosine were used to plot reference curve,FIA/FAA ratio could be calculated as the results of both the assays were expressed with a compatible activity unit.