Protective mechanism of quercetin and rutin on 2,2′-azobis(2-amidinopropane)dihydrochloride or Cu-induced oxidative stress in HepG2 cells |
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Authors: | Gyo-Nam KimYoung-In Kwon Hae-Dong Jang |
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Institution: | Department of Food and Nutrition, Hannam University, Daejeon 305-811, Republic of Korea |
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Abstract: | Protective effects of quercetin and rutin against oxidative stress were evaluated using in vitro and intracellular antioxidant assay. Quercetin showed higher peroxyl and hydroxyl radical-scavenging activity in a dose-dependent manner than did rutin in oxygen-radical absorbance capacity (ORAC). At 10 and 100 μM, quercetin had higher metal-chelating activity than rutin carrying rutinose at position C-3 and was also more efficient than rutin in reducing intracellular oxidative stress caused by peroxyl radicals and Cu2+. The protective activities of 10 and 100 μM quercetin against Cu2+-induced intracellular oxidation were 13.8% and 44.8%, respectively. Rutin showed no protective activity against Cu2+-induced oxidative stress. Quercetin showed significantly lower intracellular Cu2+-chelating activity than did 1,10-phenanthroline but offered greater protection from Cu2+-induced oxidative stress. Thus, quercetin may diffuse through the cell membrane more efficiently than rutin because quercetin does not carry rutinose, is hydrophilic, and reduces Cu2+-induced oxidative stress by scavenging radicals instead of chelating with metal ions. |
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Keywords: | Quercetin Rutin Oxidative stress Oxygen radical absorbance capacity HepG2 cells |
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