冷冻保护剂与精液比例对冷冻复苏后精子活动力的影响

目的:比较冷冻保护剂与精浆的不同比例对人类精子冷冻复苏后活动力的影响。方法:将57例志愿者的精液标本分别采用冷冻保护剂∶精液=1∶1和冷冻保护剂∶精液=1∶3的体积比进行冷冻,比较精子复苏后的前向活动率和总活动率。结果:冷冻前的前向运动精子百分率和总活动率分别为(58.60±5.57)%和(66.17±5.24)%。采用冷冻保护剂∶精液=1∶1比例进行冷冻复苏后的前向运动精子百分率和总活动率分别为(40.53±8.97)%和(51.23±9.30)%;采用冷冻保护剂∶精液=1∶3比例进行冷冻复苏后的前向运动精子百分率和总活动率分别为(44.70±8.67)%和(51.50±7.40)%。冷冻前后精子的前向运动百分率和总活动率差异有显著性(P<0.05)。两种不同比例的保护剂相比冷冻后前向运动精子百分率差异有显著性(P<0.05),而总活动率差异没有统计学意义。结论:冷冻对精子活动力损伤明显,冷冻保护剂∶精液=1∶3比例较冷冻保护剂∶精液=1∶1比例可提高冷冻后前向运动精子百分率。

Effects of Different Proportions of Cryoprotectant to Seminal Plasma on the Motility of Post-Thaw Human Sperm

Objective ： To investigate the effects of different proportions of cryoprotectant to seminal plasma on the motility of post-thaw human sperm. Methods ： Different proportions of cryoprotectant to seminal plasma （ 1 ： 1 and 1：3） were used for freezing sperm, and the forward movement and total motility rates of the frozen-thawed sperm were compared. Results ： The forward movement and total motility rates were （ 58.60 ± 5.57 ） % and （66.17 ± 5.24） % before cryopreservation. The 1 ： 1 proportion achieved post-thaw forward movement and total motility rates of （40.53 ± 8.97 ） % and （51.23 ± 9.30） %, while the 1 ： 3 （44.7 ± 8.67 ） % and （ 51.50 ±7.40） %, respectively. Significantly decreased sperm motility was observed after cryopreservation （P 〈 0.05）. Statistically significant differences were found in the forward movement but not in the total motility of the frozen-thawed sperm between the two proportions. Conclusion ： Cryopreservation causes obvious damage to human sperm. Higher proportion of cryoprotectant to seminal plasma （1：3） can improve the forward movement of post-thaw sperm as compared with the lower one （ 1 ： 1 ）.