白藜芦醇对小鼠局灶性缺血/再灌注后急性期的影响

目的探讨白藜芦醇对小鼠局灶脑缺血后急性期的影响和机制。方法90只健康♂Balb/c小鼠。随机分为单纯缺血组、白藜芦醇组及非诺贝特组,各组均为30只。采用线栓法制备小鼠大脑中动脉阻断(MCAO)模型,缺血2h,再灌注24h后,2,3,5-氯化三苯基四氮唑(TTC)法观察梗死范围和水肿程度;Evansblue染色观察血脑屏障破坏程度;RT-PCR技术检测过氧化物酶体增殖物激活受体α的mRNA水平。结果白藜芦醇和非诺贝特能够减少脑梗死体积〔(43.80±5.31)mm3、(45.57±5.63)mm3vs(63.87±9.33)mm3,P<0.01)〕,减少Evansblue外渗的体积〔(58.66±4.65)mm3、(78.72±5.78)mm3vs(102.06±6.07)mm3,P<0.01)和含量(6.40±0.92)μg.g-1、(12.47±1.24)μg·g-1vs(15.25±1.51)μg·g-1,P<0.01)〕,增强过氧化物酶体增殖物激活受体α的表达〔(0.893±0.028)、(1.117±0.042)vs(0.467±0.013),P<0.01〕。结论白藜芦醇可能通过诱导过氧化物酶体增殖物激活受体α表达增高,减轻血脑屏障的损害,从而对脑缺血/再灌注损伤起到保护作用。

Resveratrol's effect on acute phases of focal cerebral ischemic injury in mice

Aim To discuss the influence of resveratrol on acute phases of focal cerebral ischemic injury in mice and possible mechanisms.Methods Ninety Balb/c healthy male mice,weighting 18~22 g,were randomly divided into three groups:resveratrol group,fenofibrate group and ischemia group.There were thirty mice in each group.The ischemic model of mice was induced with an 8/0 nylon monofilament coated with silicone hardener mixture via the internal carotid artery.24 hours after reperfusion,the infarct area was shown by 2,3,5-triphenyl tetrazolium chlorid(TTC)staining;Evans blue staining was used for showing the damage of blood brain barrier and the express of peroxisome proliferator activated receptor(PPAR)alpha was shown by RT-PCR.Results The infarct volume in resveratrol group and fenofibrate group were lower thanischemia group〔(43.80±5.31)mm3,(45.57±5.63)mm3 vs(63.87±9.33)mm3,P<0.01)〕.In resveratrol group and fenofibrate group,the extent and quality of Evans blue extravasation were reduced compared with ischemia group〔(58.66±4.65)mm3,(78.72±5.78)mm3 vs(102.06±6.07)mm3,P<0.01〕 and(6.40±0.92)μg·g-1,(12.47±1.24)μg·g-1 vs(15.25±1.51)μg·g-1,P<0.01)〕,PPARα mRNAs were prominently expressed compared with ischemia group(0.893±0.028,1.117±0.042 vs 0.467±0.013,P<0.01).Conclusion The study suggests that resveratrol has protective effects against acute ischemic stroke,which could be attributed to its property increasing PPARα.Thus,resveratrol may be a potential agent for the treatment of neuronal injury associated with stroke.