腭 肺及鼻咽上皮克隆蛋白和Toll样受体-4在鼻息肉组织中的表达及相关性分析

目的运用蛋白印迹法明确腭、肺及鼻咽上皮克隆(PLUNC)蛋白和TLR-4在鼻息肉和正常鼻黏膜组织之间的表达差异和相关性,进一步探讨二者在鼻息肉病因学及疾病进展中的作用。方法采用蛋白印迹法分别半定量检测45例鼻息肉和15名正常鼻黏膜组织中PLUNC蛋白和TLR-4的表达情况,并分析二者在鼻息肉组织中表达的相关性。结果①鼻息肉组与对照组鼻黏膜组织之间PLUNC蛋白和TLR-4表达差异均有统计学意义(P<0.05),鼻息肉组织中TLR-4高表达,PLUNC蛋白低表达;②鼻息肉组PLUNC蛋白和TLR-4表达呈负相关性(r=-0.384,P<0.05)。结论感染性因素在鼻息肉的发病过程中起着重要的作用,感染性因素的存在(特别是革兰阴性菌)使得鼻黏膜TLR-4表达量增高,鼻黏膜长期处于慢性炎症反应使得PLUNC表达分泌下降,可能促进了鼻息肉形成。

Correlation between expression of PLUNC and Toll-like receptor 4 in nasal polyps

Objective To investigate the difference in and correlation of PLUNC and toll-like receptor 4 （TLR-4） expression in nasal polyps tissue and normal nasal mucosa by immunoblotting techniques, and to determine the roles of these two markers in the pathogenesis of nasal polyps. Methods Forty-five specimens of nasal polyps and 15 of normal nasal mucosa were biopsied for Western blotting for semiquantitative assessment of PLUNC and TLR-4 ex-pression. The correlation between the two markers was also evaluated. Data were analyzed by using the SPSS 17.0 software. Results Compared with controls, the expression of TLR-4 was significantly increased and PLUNC reduced in nasal polyps （both P〈0.05）. There was negative correlation between PLUNC and TLR-4 in nasal polyps （r=-0.384, P〈0.05）. Conclusion Infective factors may play an important role in the pathogenesis of nasal polyps. The existence of infective factors （esp. Gram-negative bacteria） may increase TLR-4 expression in the nasal mucosa, which suppress PLUNC secretion leading to the development of nasal polyps.