OK-432肿瘤细胞疫苗对DBA/2小鼠舌癌生长抑制作用 |
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引用本文: | 甘世新,;武峰,;姚景春.OK-432肿瘤细胞疫苗对DBA/2小鼠舌癌生长抑制作用[J].中国药物与临床,2014(7):870-872. |
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作者姓名: | 甘世新 ;武峰 ;姚景春 |
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作者单位: | [1]山西省肿瘤医院头颈二科,太原030013; [2]山西医科大学口腔医院修复科,太原030013; |
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基金项目: | 基金项目:国家自然科学基金(81141091) |
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摘 要: | 目的利用DBA/2小鼠移植舌癌荷瘤模型,探究OK-432肿瘤细胞疫苗对舌癌的抑制作用。方法以戊二醛为交联剂,使KLN-205细胞与OK-432产生交联,制备OK-432肿瘤细胞疫苗;将DBA/2小鼠随机分为4组,每组20只,分别右侧腹部皮下注射0.9%氯化钠注射液、OK-432、灭活的KLN-205细胞、OK-432疫苗,接种KLN-205肿瘤细胞后观察对比舌癌瘤体大小,并用统计学分析其结果。结果肿瘤大小除KLN-205组与对照组间差异没有统计学意义(P=0.094)外,其余各组间差异均有统计学意义,其中OK-432疫苗组肿瘤体积均小于其他3组。结论 OK-432肿瘤细胞疫苗能抑制小鼠舌癌的生长,具有一定的抗肿瘤效应。
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关 键 词: | 癌症疫苗 DBA/2小鼠 KLN-205细胞 |
OK-432 tumor cell vaccine inhibits tongue cancer growth in DBA/2 mice |
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Institution: | GAN Shixin, WU Feng, YAO Jingchun (Department of Head and Neck, Shanxi Cancer Hospital, Taiyuan 030013, China) |
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Abstract: | Objective To investigate the inhibitory effects of OK-432 tumor cell vaccine on tongue cancer growth in DBA/2 mice by using the tongue cancer xenograft models. Methods The OK-432 tumor cell vaccine was prepared by inducing conjugation of OK-432 to squamous cell carcinoma (KLN-205) with 0.2% glutaraldehyde (GA). The DBA/2 mice were randomly allocated to receive subcutaneous injection of saline ( n=20), OK-432 (n=20), inacti-vated KLN-205 cells (n=20) and OK-432 tumor cell vaccine (n=20) on the right abdomen. This allowed assessment of the tumor size before and after KLN-205 cell inoculation. Results The tumor size was significantly different among the four groups except for OK-432 vaccine group compared with control group (P=0.094). Inoculation with OK-432 vaccine resulted in marked reduction in the tumor size compared with the remaining three groups. Conclusion OK-432 tumor cell vaccine can inhibit the growth of tongue cancer in mice, suggesting the potential anti-tumor effects. |
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Keywords: | Cancer vaccines DBA/2 mice KLN-205 cell |
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