钩藤碱对苯丙胺依赖大鼠脑内氨基酸类神经递质含量的影响

目的:观察钩藤碱对苯丙胺依赖大鼠脑内氨基酸类神经递质含量的影响。方法:采用条件性位置偏爱实验和脑电超慢涨落分析技术。结果:氯胺酮和钩藤碱均能消除苯丙胺导致的位置偏爱效应;苯丙胺使大鼠呈现完全偏离的“脑功能平衡图”,氯胺酮和钩藤碱可使其恢复至平衡态;苯丙胺导致大鼠脑内抑制性氨基酸含量明显下降(P<0.01),兴奋性氨基酸含量明显上升(P<0.01),氯胺酮和钩藤碱均可恢复其至正常水平,钩藤碱本身对这些均无影响。结论:钩藤碱可调整苯丙胺引起的大鼠脑内氨基酸神经递质含量的异常变化。

EFFECT OF RHYNCHOPHYLLINE ON AMINO ACID NEUROTRANSMITTER CONTENT IN BRAIN OF AMPHETAMINE-DEPENDENT RATS

Objective:To explore the effect of rhynchophylline on amino acid neurotransmitter content in the brain of amphetamine-dependent rats.Methods:The staying time in white compartment of rats was determined in conditioned place preference(CPP)experiment.Encephalofluogram technology was used to detect the brain function and inhibitory amino acids,excitatory amino acids content in each group.Results:Ketamine and rhynchophylline both could eliminate amphetamine induced CPP effect;rhynchophylline couldn't induce CPP in normal rats.Brain electrical activity maps of normal control group appeared as “brain function balance picture”,but the model group showed almost disrupted brain function state,while the ketamine group and rhynchophylline group and rhynchophylline + saline group showed similar brain function state to the normal control group.In comparison with normal control group,ketamine group,rhynchophylline group and rhynchophylline + saline group,the inhibitory amino acids content in the brain of amphetamine model group was obviously lower(P<0.01),but excitatory amino acids content was significantly higher(P<0.01).Conclusion:Rhynchophylline can adjust the abnormal content of amino acid neurotransmitter induced by amphetamine.Brain electrical activity map and amino acid neurotransmitter participate in the electrophysiological mechanism of antagonistic effect of rhynchophylline on amphetamine-dependent rats.