首页 | 本学科首页   官方微博 | 高级检索  
     


Recovery from paralysis in adult rats using embryonic stem cells
Authors:Deshpande Deepa M  Kim Yun-Sook  Martinez Tara  Carmen Jessica  Dike Sonny  Shats Irina  Rubin Lee L  Drummond Jennifer  Krishnan Chitra  Hoke Ahmet  Maragakis Nicholas  Shefner Jeremy  Rothstein Jeffrey D  Kerr Douglas A
Affiliation:Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287-6965, USA.
Abstract:OBJECTIVE: We explored the potential of embryonic stem cell-derived motor neurons to functionally replace those cells destroyed in paralyzed adult rats. METHODS: We administered a phosphodiesterase type 4 inhibitor and dibutyryl cyclic adenosine monophosphate to overcome myelin-mediated repulsion and provided glial cell-derived neurotrophic factor within the sciatic nerve to attract transplanted embryonic stem cell-derived axons toward skeletal muscle targets. RESULTS: We found that these strategies significantly increased the success of transplanted axons extending out of the spinal cord into ventral roots. Furthermore, transplant-derived axons reached muscle, formed neuromuscular junctions, were physiologically active, and mediated partial recovery from paralysis. INTERPRETATION: We conclude that restoration of functional motor units by embryonic stem cells is possible and represents a potential therapeutic strategy for patients with paralysis. To our knowledge, this is the first report of the anatomical and functional replacement of a motor neuron circuit within the adult mammalian host.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号