人成釉细胞瘤中端粒酶反转录酶启动子区的DNA甲基化

目的研究成釉细胞瘤（ameloblastoma，AB）中hTERT启动子区的DNA甲基化，并探讨其生物学意义。方法选取新鲜标本AB12例，同时用11例正常黏膜做对照观察，用甲基化特异PCR检测上述组织中人类端粒酶反转录酶（human telomerase reverse transcripase，hTERT）启动子区的DNA甲基化。结果AB、正常黏膜中hTERT启动子区的DNA非甲基化阳性分别为4例（4／12）和6例（6／11）。AB、正常黏膜中hTERT启动子区的DNA甲基化阳性分别为11例（11／12）和3例（3／11），其中4例AB和1例正常黏膜同时表现hTERT启动子区DNA的甲基化和非甲基化。结论AB的hTERT启动子区的DNA甲基化比正常黏膜常见且有意义；hTERT启动子的甲基化可能对hTERT基因起调节作用。

DNA mthylation of human telomerase reverse transcripase promoter region in human amelobalstoma

OBJECTIVE: To study the DNA methylation of human telomerase reverse transcripase (hTERT) promoter in amelobalstoma (AB) and investigate its clinical biological significance. METHODS: DNA methylation of hTERT promoter in 12 cases of AB and 11 cases of normal oral mucosa were detected by methylation-specific PCR. RESULTS: The positive cases of unmethylated hTERT promoter region in AB and normal oral mucosa were 4 (4/12) and 6 (6/11), respectively (P < 0.001). The positive cases of methylated hTERT promoter region in AB and normal oral mucosa were 11 (11/12) and 3 (3/11), respectively. Four cases of AB and 1 case of normal oral mucosa showed both DNA methylation and unmethylation of hTERT promoter region in the same time. CONCLUSIONS: DNA methylation of of hTERT promoter region in AB was more common than that in normal oral mucosa. The DNA methylation in hTERT promoter maybe regulate hTERT gene expression.