口腔鳞癌间质微血管的特征及意义

目的观察口腔鳞癌间质微血管的空间分布和形态学特征，分析它们与临床病理学参数间的关系，探讨抗血管生成治疗在口腔鳞癌治疗中的作用。方法应用双标免疫组织化学及计算机图像分析技术，检测和分析62例口腔鳞癌及30例癌旁正常组织和10例正常口腔黏膜的微血管分布及形态。结果口腔鳞癌间质微血管与癌旁和正常口腔黏膜微血管相比，前者具有血管密度高、不成熟、形态不规则、血管周长较小的特点（P〈0．01）；口腔鳞癌癌灶边缘的血管与癌灶内血管相比，血管密度更高（P〈0．01）、更不成熟（P〈0．05）；微血管密度与肿瘤的淋巴结转移有关（P〈0．01）。结论口腔鳞癌间质微血管与正常口腔组织血管比较，差异有统计学意义，该差异有利于抗血管生成治疗的应用。癌灶边缘可能是抗血管生成治疗的重要靶区。微血管密度可望成为判断肿瘤恶性程度的一项指标。

The characteristics and significance of microvessel in oral squamous cell carcinoma

OBJECTIVE: To investigate the characteristics of distribution status of maturation and morphology of the microvessel in oral squamous cell carcinoma (OSCC) and analyze the correlations between the characteristics and clinicopathologic parameters. METHODS: The microvessel in 62 cases of OSCC, 30 cases of adjacent normal tissue and 10 cases of normal oral mucosa were detected by double-labeling immunohistochemistry technique. Image analysis mucosa, the microvascular density (MVD) was higher while vessel maturation system was also used for the analysis RESULTS: Compared with adjacent normal tissues and normal oral index (VMI), vascular form factor (VFF) and vascular perimeter (VP) were lower in OSCC with significant difference (P < 0.01). The MVD was higher (P < 0.01) and VMI was lower (P < 0.05) in peritumoral site than those in intratumoral site. The MVD were correlated significantly with lymph node metastasis (P < 0.01). CONCLUSIONS: The microvessels in OSCC were significantly different from those in normal oral tissues. The differences may contribute to the application of antiangiogenic therapy, and the peritumoral area in OSCC may become a target site of the therapy. The MVD may be hopefully used as an indicator of tumor malignancy.