过量氟对大鼠切牙碱性成纤维细胞生长因子表达的影响

目的 研究过量氟对大鼠切牙碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）表达的影响，从分子水平探讨氟牙症的发病机制。方法将20只Wistar大鼠按随机数字表法随机分为两组：对照组（饮用蒸馏水）和实验组（饮用100mg／L氟化水），复制氟牙症动物模型，8周末处死动物，获取切牙标本，免疫组化染色观察bFGF在大鼠切牙的表达定位及其在对照组与实验组切牙表达的变化。结果bFGF在分泌期成釉细胞、成牙本质细胞中均呈阳性表达。实验组bFGF的表达明显弱于对照组，差异有统计学意义（P〈0．01）。结论过量氟可抑制bFGF的表达，从而影响上皮与间充质之间的相互介导作用，导致釉质发育障碍。

Effect of fluorosis on the expression of basic fibroblast growth factor in rat incisors

OBJECTIVE: To investigate the effects of overdose fluoride on the expression of basic fibroblast growth factor (bFGF) in rat incisors. METHODS: Twenty Wistar rats were randomly divided into 2 groups: group I (control group) distilled water was given; group II (experimental group) 100 mg/L NaF was given. The rats were killed at the end of 8 th week. Immunohistochemical staining was used to study the expression of bFGF in rat incisors. RESULTS: Immunohistochemical results demonstrated the presence of bFGF in ameloblasts, odontoblasts of rat incisors. The expression of bFGF was reduced in group II (P < 0.01). CONCLUSIONS: Overdose fluoride inhibits the expression of bFGF and affects the interaction between dental epithelium and dental mesenchyme, which leads to the enamel demineralization.