香附四物汤对小鼠脾淋巴细胞增殖转化影响的效应物质基础研究

目的:评价香附四物汤及各分离化学部位对小鼠脾淋巴细胞增殖转化的影响。方法:采用小鼠脾淋巴细胞增殖与转化实验方法,对香附四物汤及各分离化学部位进行免疫相关的生物效应评价;采用HPLC对效应显著部位的化学成分进行定性定量分析。结果:香附四物汤、醇沉上清液部位及其经大孔吸附树脂吸附后30%乙醇洗脱部位具有明显促进小鼠脾淋巴细胞增殖与转化作用,其中,30%乙醇洗脱部位的生物效应显著;对30%效应部位进行定性定量分析表明其主要组成及其含量分别为芍药内酯苷5.973%,芍药苷5.902%,阿魏酸1.374%,洋川芎内酯I 0.014%。结论:香附四物汤对小鼠脾淋巴细胞增殖转化影响的主要效应物质基础是含有芍药内酯苷、芍药苷、阿魏酸和洋川芎内酯I等的30%乙醇洗脱部位。

Study on Mice Spleen Lymphocyte Proliferation and Transformation Effect and Chemical Constituents of Xiangfu Siwu Decoction

Objective: To evaluate the mice spleen lymphocyte proliferation and transformation effect of Xiangfu Siwu decoction(XFSWD) and explore the bioactive constituents. Method: The samples were prepared by macroporous adsorptive resins. mice spleen lymphocyte proliferation and transformation models were adopted to evaluate the immune related activities of XFSWD and its different separated fractions in vitro. The chemical constituents in the most active fraction were identified and determined by HPLC. Result: By mice spleen lymphocyte proliferation and transformation experiments, XFSWD, XFSW-3 and XFSW-7 showed high effect. Four main compounds including albiflorin, peoniflorin, ferulaic acid, senkyunolide I in active fraction XFSW-7 were identified and quantified by HPLC-DAD. The contents were 5.973%, 5.902%, 1.374% and 0.014%, respectively. Conclusion: Albiflorin, peoniflorin, ferulaic acid, senkyunolide I were the potential active constituents. XFSWD had the common material basis.