水分胁迫下白术过氧化物酶同工酶的变化分析

目的：研究不同处理条件下，水分胁迫对不同形态类型白术叶和花蕾中过氧化物酶（POD）同工酶的影响。方法：采用盆栽控制试验，利用垂直板聚丙烯酰胺凝胶电泳分析技术进行同工酶分析。结果：（1）在一定水分胁迫范围内，白术叶片的POD同工酶的活性及酶带数目随水分亏缺的加剧而升高和增多，但过度水分胁迫时，酶的活性有所下降且数目减少；（2）不同形态类型白术叶片的POD同工酶在水分胁迫下的变化有所不同，其中中间型白术的耐旱性略高于阔叶和狭叶白术；（3）叶片中的POD同工酶的表达较花蕾中的丰富，且酶带的变化也较大，研究水分胁迫对白术POD同工酶的影响以取叶片作分析材料为佳。结论：研究水分胁迫下白术体内POD同工酶的变化，可为白术抗旱性提供生理生化参考依据。

Effects of Water Stress on POD Isozyme in Atractylodes macrocephala Koidz.

Objective:To study the effects of soil water stress on POD isozyme in leaves and flowers of three different types of Atractylodes macrocephala Koidz..Methods: Pot control was adopted in the experiments and vertical slab polyacrylamide gel electrophoresis technique was utilized in the POD analysis.Results: (1)Within a certain rang of soil water stress, the activities and numbers of POD isozyme in leaves increased with the decrease of soil water content, but decreased when reached the over-stress level; (2)There were different changes of POD isozyme in leaves in different types under the soil water stress, and the drought tolerance of mid-type was better than others; (3) The POD isozyme in leaves was more complicated than in flowers, and also the changes under the soil water stress was. To study the effects of soil water stress on POD isozyme on Atractylodes macrocephala Koidz., the leaves was the better experimental material than flowers. Conclusion:This study could be applied to analysis the drought resistance of Atractylodes macrocephala Koidz..