甲醛和苯对小鼠睾丸总抗氧化能力和ATP酶及钙的影响

目的探讨甲醛和苯对小鼠睾丸总抗氧化能力和ATP酶及钙的影响及二者联合毒效应的类型。方法采用昆明种雄性小鼠54只随机等分为9组，按3×3析因设计要求设9个染毒组，甲醛和苯染毒剂量分别为0、5、10mg／kg和0、100、400mg／kg，连续灌胃染毒5d，35d后处死小鼠取睾丸制作匀浆液测Na^＋-K^＋-ATP、Ca^＋-Mg^＋-ATP酶活性、Ca^2＋含量和总抗氧化能力。结果甲醛和苯各染毒组小鼠睾丸总抗氧化能力、Na^＋-K^＋-ATP、Ca^2＋-Mg^＋-ATP酶活性和Ca^2＋含量与对照组比较差异有显著性（P〈0．01），并有量-效关系，甲醛和苯之间的联合毒性效应是协同作用。结论甲醛和苯能抑制小鼠睾丸Na^＋-K^＋-ATP、Ca^2＋-Mg^2＋-ATP酶的活性、降低总抗氧化能力和提高Ca^2＋的含量，二者对这些指标的影响表现为协同毒效应，这可能是甲醛和苯对生殖功能损伤的机制。

Effects of Formaldehyde and Benzene on Total Antioxidant Capacity,ATPase and Ca2 in Testicle of Mice

Objective To study the combined toxicity effects of formaldehyde and benzene on the total antioxidant capacity, ATPase, and Ca^2＋ in the testicle of mice. Methods Fifty- four Kunming male mice were randomly divided into 9 treated groups. Various doses of formaldehyde （0,5, and 10 mg/kg）, benzene （0, 100, and 400 mg/kg） separately or formaldehyde plus benzene were given into 9 groups of mice by gastric gavage for .5 successive days. After 3.5 days, these mice were sacrificed to measure the activity of Na^＋ - K^＋ - ATPase, the activity of Ca^2＋ - Mg^2＋ - ATPase, the content of Ca^2＋ and the total antioxidant capacity in testicle of mice. 3 × 3 factorial analysis was used in the experiment. Results Compared with the negative control group, the activity of Ca^2 - Mg^2＋ - ATPase, the activity of Na^＋ - K^＋ - ATPase, the total antioxidant capacity and the content of Ca^2 ＋ of the mice testicles among treatment groups presented a significant dose - effect relationship and significance of difference （P〈 0.01 ）. And the joint toxic effect exhibited synergistic effect. Conclusions In the testicle of mice, the activity of Na^＋ - K^＋ - ATPase, the activity of Ca^2＋ - Mg^2＋ - ATPase and the total antioxidant ca- pacity can be restrained, while the content of Ca^26 can be increased by formaldehyde and benzene, which shows the testicular cells of mice can be enhanced to damage jointly by formaldehyde and benzene.