|
|||||
|
|
| 生殖细胞核因子及Oct-4在P 19细胞系诱导分化中的表达 | |
| 引用本文： | 王菊,熊承良,高启云,李红钢,王苗,赵凯,周宗瑶. 生殖细胞核因子及Oct-4在P 19细胞系诱导分化中的表达[J]. 解剖学报, 2013, 44(3): 349-352. DOI: 10.3969/j.issn.0529-1356.2013.03.011 |
| 作者姓名： | 王菊 熊承良 高启云 李红钢 王苗 赵凯 周宗瑶 |
| 作者单位： | 1.石河子大学医学院组织学胚胎学教研室，新疆 石河子 832002; 2.华中科技大学同济医学院计划生育研究所/生殖医学中心，武汉 430030 |
| 摘 要： | 目的 体外培养未成熟畸胎瘤细胞（P19细胞）并诱导分化，检测生殖细胞核因子（GCNF）及Oct-4在P 19细胞诱导分化前后的表达。 方法 用全反式维甲酸（ATRA）诱导P 19细胞分化，通过间接免疫荧光染色和RT-PCR方法检测GCNF及Oct-4在P 19细胞诱导分化前后的表达。 结果 Oct-4在P 19细胞诱导前呈强阳性表达，诱导2 d后表达下降；而GCNF在P 19细胞诱导前呈阴性表达，诱导2 d后呈阳性表达。 结论 GCNF参与了恶性生殖细胞肿瘤的体外分化。GCNF可能是通过下调Oct-4基因的表达参与了恶性生殖细胞肿瘤的分化。 |
| 关 键 词： | Oct-4 生殖细胞核因子 P19细胞 分化 间接免疫荧光 反转录-聚合酶链反应 |
| 收稿时间： | 2012-06-18 |
Expression of germ cell nuclear factor and Oct-4 before and after in vitro differentiation of mouse P19 Cell line |
|
| WANG Ju,XIONG Cheng-liang GAO Qi-yun LI Hong-gang WANG Miao ZHAO Kai ZHOU Zong-yao. Expression of germ cell nuclear factor and Oct-4 before and after in vitro differentiation of mouse P19 Cell line[J]. Acta Anatomica Sinica, 2013, 44(3): 349-352. DOI: 10.3969/j.issn.0529-1356.2013.03.011 | |
| Authors: | WANG Ju XIONG Cheng-liang GAO Qi-yun LI Hong-gang WANG Miao ZHAO Kai ZHOU Zong-yao |
| Affiliation: | 1. Department of Histology and Embryology, School of Medicine, Shihezi University, Xinjiang Shihezi 832002, China;;2. The Family Planning Institute, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China |
| Abstract: | Objective To detect the expressions of Oct-4 and germ cell nuclear factors(GCNF) before and after inducing P19 cells to differentiation. Methods The P19 cells were induced to differentiation with ATRA treatment.The immunofluorescence staining and RT-PCR techniques were used to detect the expression of GCNF and Oct-4 before and after differentiation of mouse P19. Results The intensities for Oct-4 immunofluorescence positive staining were stronger in non-differentiated P19 cells than that in the differentiated P19 cells. The intensities for GCNF immunofluorescence positive staining were weaker in non-differentiate P19 cells than that in the differentiated P19 cells. Conclusion GCNF is involved in the in vitro differentiation progress of the malignant germ cell tumors. GCNF be inhibited through the Oct-4 gene expression in vitro differentiation of P19 cells. |
| Keywords: | Oct-4 Germ cell nuclear factor P19 cell Differentiation Indirect immunofluorescence RT-PCR |
| 点击此处可从《解剖学报》浏览原始摘要信息 | |
| 点击此处可从《解剖学报》下载全文 | |