| 巢式PCR法检测髓系白血病患者外周血人类疱疹病毒—6的NDA |
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| 引用本文： | 卢育洪,陈少华.巢式PCR法检测髓系白血病患者外周血人类疱疹病毒—6的NDA[J].中国实验血液学杂志,2000,8(3):189-191. |
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| 作者姓名： | 卢育洪 陈少华 |
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| 作者单位： | [1]暨南大学医学院附属第一医院血液内科 [2]血液病研究室 |
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| 摘 要： | 人类疱疹病毒－６型（ｈｕｍａｎ ｈｅｒｐｅｓｖｉｒｕｓ－６，ＨＨＶ－６）是一种常见的病毒，与多种人类疾病有关。由于研究过程所采取方法不同，对于白血病病人感染ＨＨＶ－６情况的报道也不一致。本研究首次运用巢式ＰＣＲ检测了３８例髓系白血病病人外周血单个核细胞中的ＨＨＶ－６ ＤＮＡ，其中急性髓性白血病（ＡＭＬ）３０例和慢性粒细胞白血病（ＣＭＬ）８例。结果显示：与下沉对照ＨＨＶ－６的ＮＤＡ检出率相比，ＡＭＬ
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| 关 键 词： | 人类疱疹病毒－6 髓系 白血病 外周血 巢式PCR |
| 修稿时间： | 2000年1月25日 |
Detection of Human Herpesvirus-6 DNA in Peripheral Blood Cells of Patients with Myelogenous Leukemia by Nested PCR |
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| LU Yuhong,ZHOU Yubing,CHEN Shaohua,YANG Lijian,LI Yangqiu.Detection of Human Herpesvirus-6 DNA in Peripheral Blood Cells of Patients with Myelogenous Leukemia by Nested PCR[J].Journal of Experimental Hematology,2000,8(3):189-191. |
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| Authors: | LU Yuhong ZHOU Yubing CHEN Shaohua YANG Lijian LI Yangqiu |
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| Abstract: | Human herpesvirus-6(HHV-6) is largly disseminated in human population, which may relate to many diseases. Due to different methods previously used, the results of HHV-6 DNA detections in patients with leukemia are not identical. With a control-study, HHV-6 DNA in the blood cells from 38 cases, including 30 cases with acute myeloid leukemia and 8 cases with chronic myelogenous leukemia was detected by nested PCR. The results showed that HHV-6 DNA positive rate(63%) in patients with AML was lower than that in controls, whereas no significant difference between patients with CML(75%) and controls(92%) was shown. The clinical importance of HHV-6 infections in myelogenous leukemia should continue to be investigated later. |
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| Keywords: | human herpesvirus-6myelogenous leukemiaperipheral bloodnested PCR |
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