有机酸对人血管内皮细胞保护作用的机制研究

目的: 研究氯原酸(CHA)、抗坏血酸(AA)、柠檬酸(CA)和苹果酸(MA)四种有机酸(OA)对单核细胞趋化蛋白1(MCP-1)和单核细胞聚集刺激因子(M-CSF)的影响及其抗氧化作用,探讨有机酸保护人血管内皮细胞的作用机制。 方法: 在体外原代培养人脐静脉内皮细胞(EC)中,分别加入不同剂量(10,20,40 mg/L)的CHA、AA、CA和MA,以及氧化型低密度脂蛋白(ox-LDL,100 mg/L),观察四种OA对ox-LDL引起人血管EC损伤的保护作用。另观察单纯CHA和AA(40 mg/L)对EC的直接作用。 结果: (1)ox-LDL组的TBARS值明显升高,是正常LDL组的14.85倍;加OA各组TBARS值有不同程度的降低,并呈明显的剂量效应关系,其中CHA和AA作用较强,CA和MA作用较弱。(2)ox-LDL组MCP-1和M-CSF较LDL组显著升高;加OA组(40 mg/L)MCP-1和M-CSF分别显著低于ox-LDL组;单纯CHA和AA组分别低于空白对照组。 结论: OA对EC的保护作用与其抗氧化作用有关,而且可能与对EC分泌MCP-1和M-CSF的影响有关。

STUDY ON THE PROTECTIVE MECHANISM OF ORGANIC ACIDS IN HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

Objective: To examine the effects of four organic acids (OA), namely chlorogenic acid (CHA), ascorbic acid (AA), citric acid (CA) and malic acid (MA), on monocyte chemotactic protein-1 (MCP-1) and monocyte colony stimulating factor (M-CSF), as well as on antioxidant function in human vascular endothelial cells (EC). Methods: Original human umbilical vein EC were cultured and incubated for 12 h respectively with ox-LDL, in the presence of CHA, AA, CA and MA at different concentrations(10, 20, 40 mg/L), to study the protective effect on human vascular EC and its mechanism . Results: (1) TBARS value of oxLDL group was 14.85 times higher than that of normal LDL group which was not different with blank group. TBARS values of the OA oxLDL group were lower at different extents when compared with ox-LDL group, showing dose-effect response. The inhibitory effects of CHA and AA were better than those of CA and MA. (2) MCP-1 and M-CSF of ox-LDL group were higher than those of blank group. Both MCP-1 and M-CSF of OA ox-LDL groups statistically decreased when compared with ox-LDL group; MCP-1 and M-CSF of single CHA or AA (40 mg/L)group were lower than those of blank group respectively. Conclusion: The protective effects of OA on human vascular EC were contributed to their antioxidant activities, probably through MCP-1 and M-CSF .