东莨菪碱对吗啡依赖大鼠条件位置性偏爱激活的抑制作用

目的:观察东莨菪碱(Scopolamine,Sco)对吗啡(Morphine,Mor)依赖大鼠条件位置性偏爱激活的抑制作用。方法:以剂量递增连续皮下(SC)给吗啡6天建立吗啡诱导大鼠条件位置性偏爱(CPP)模型,第7天用生理盐水替代吗啡训练大鼠10天,使形成的CPP逐渐消退,单次SC4mg/kg吗啡激发消退的CPP。部分大鼠在注射吗啡前30分钟分别腹腔注射(ip)Sco(1mg/kg、2mg/kg、3mg/kg)。观察东莨菪碱对吗啡依赖大鼠在伴药箱停留时间的变化。结果:与Mor依赖组相比在SC4mg/kgMor引燃刺激前30min应用Sco1mg/kg、2mg/kg、3mg/kg均可以使大鼠在伴药箱停留时间缩短(P<0.05)。结论:Sco一定程度上抑制Mor引燃的Mor依赖大鼠的条件位置性偏爱激活。

The Inhibition Efficacy of Scopolamine on Reactivation of conditioned place preference in Morphine-Dependent Rats

Objective:To study inhibitory efficacy of scopolamine on reactivation of conditioned place preference(CPP)in morphine dependent rats.Methods:Morphine was administered by subcutaneous injection at gradually increasing dose(from10mg/kg to 60 mg/kg)for 6 days to establish morphine conditioned place preference .From day 7,the rats were administered normal sodium instead of morphine for 10 days to induce CPP extinction.The rats were given a single priming injection of morphine 4 mg/kg to reinstate the morphine CPP,some rats were treated by ip scopolamine(1mg/kg,2mg/kg,3mg /kg)prior to priming injection of morphine.Results:After priming injection of morphine 4 mg/kg,the time spent on the drug paired side was significantly reduced because of the treatment with scopolamine,compared with morphine group(P<0.05).Conclusion:Scopolamine has some inhibitory effects on reactivation of conditioned place preference in morphine-dependent rats.