THY1基因在卵巢上皮性癌组织中的表达

目的 检测人正常卵巢组织、卵巢浆液性囊腺瘤和浆液性囊腺癌组织中THY1蛋白的表达,探讨其表达变化与卵巢浆液性囊腺癌患者临床病理因素间的关系.方法 应用免疫组化法,检测25例人正常卵巢组织、25例卵巢浆液性囊腺瘤组织和53例浆液性囊腺癌组织中THY1蛋白的表达.结果 THY1蛋白在正常卵巢、卵巢浆液性囊腺瘤和浆液性囊腺癌组织中的阳性表达率分别为60.0%(15/25)、72.0%(18/25)和34.0%(18/53),IOD值分别为288 449.2±60 087.3、271 655.6±66 588.7和252 087.6±45 559.4.卵巢浆液性囊腺癌中,THY1蛋白的阳性表达率明显低于正常卵巢和卵巢浆液性囊腺瘤组织(P<0.05),且THY1蛋白的表达量与卵巢浆液性囊腺癌的手术-病理分期、组织学分级及淋巴结转移呈显著负相关(均P<0.05).结论 THY1基因的缺失可能与卵巢浆液性囊腺癌的发生、发展相关.

Expression of THY1 Gene in Epithelial Ovarian Cancer

Objective To detect the expession of THY1 in ovarian serous cystadenocareinoma tissues. Methods Immunohistochemistry was performed to detect the expression of THY1 gene in formalin-fixed, paraffin-embedded specimens of normal ovaries (n=25), ovarian serous cystadenoma (n=25), and serous cystadenocarcinoma (n=53). The correlation of THY1 expression with clinicopathological parameters was statistically analyzed. Results The positive expression rates of THY1 protein in normal ovaries, ovarian serous cystadenomas and ovarian serous cystadenocareinomas were 60.0% (15/25), 72.0% (18/25) and 34.0% (18/53), respectively. The values of IOD of THY1 protein expression were 288 449.2±60 087.3, 271 655.6±66 588.7 and 252 087.6±45 559.4, respectively. The expression of THY1 protein was significantly down-regulated in ovarian serous eystadenocarcinoma tissues compared with that in normal ovarian tissues and ovarian serous cystadenoma tissues (P<0.05). THY1 expression was negatively correlated with surgical-pathological staging, histological differentiation and lymph node involvement (P<0.05). Conclusion The decreased level of THY1 expression may be related with the occurrence and development of ovarian serous cystadenocarcinoma.